UniProt Accession

 COTL1_HUMAN; Q14019; B2RDU3; D3DUL9; Q86XM5 

Genbank Protein ID

 L54057; BT006968; AK315675; CH471114; CH471114; BC010039; BC010884; BC016702; BC042970; BC053682 

Genbank Nucleotide ID

 AAA88022.1; AAP35614.1; BAG38040.1; EAW95476.1; EAW95477.1; AAH10039.1; AAH10884.1; AAH16702.1; AAH42970.1; AAH53682.1 

Protein Name

 Coactosin-like protein 

Protein Synonyms/Alias

  

Gene Name

 COTL1 

Gene Synonyms/Alias

 CLP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MATKIDKEACR	acetylation	[1]
4	****MATKIDKEACR	ubiquitination	[2, 3]
7	*MATKIDKEACRAAY	acetylation	[3]
7	*MATKIDKEACRAAY	ubiquitination	[2]
72	TTGDAMSKRSKFALI	acetylation	[4]
72	TTGDAMSKRSKFALI	ubiquitination	[2, 3, 5]
102	GTDKTLVKEVVQNFA	acetylation	[6]
102	GTDKTLVKEVVQNFA	ubiquitination	[2, 5]
110	EVVQNFAKEFVISDR	acetylation	[6]
110	EVVQNFAKEFVISDR	ubiquitination	[2, 5]
126	ELEEDFIKSELKKAG	acetylation	[6]
130	DFIKSELKKAGGANY	ubiquitination	[2]
131	FIKSELKKAGGANYD	ubiquitination	[2]

Reference

 [1] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis. 

Sequence Annotation

 DOMAIN 2 130 ADF-H.
 REGION 66 75 Flexible and important for F-actin
 MOD_RES 102 102 N6-acetyllysine.
 MOD_RES 126 126 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Actin-binding; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Membrane; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 142 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0003779; F:actin binding; IDA:UniProtKB.
 GO:0050832; P:defense response to fungus; IEA:Compara. 

Interpro

 IPR002108; Actin-bd_cofilin/tropomyosin. 

Pfam

 PF00241; Cofilin_ADF 

SMART

 SM00102; ADF 

PROSITE

 PS51263; ADF_H 

PRINTS