CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006097
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein SSH4 
Protein Synonyms/Alias
 Multicopy suppressor of leflunomide protein 4; Suppressor of SHR3 null mutation protein 4 
Gene Name
 SSH4 
Gene Synonyms/Alias
 MLF4; YKL124W; YKL529 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
99TLHSLFGKKHSGILLubiquitination[1]
145VELYIRAKEFQMMSPubiquitination[1]
171SDDQQFIKDRGIQSYubiquitination[1]
367REALSEGKDIEVAKDubiquitination[1, 2]
373GKDIEVAKDLQRVHDubiquitination[1]
435AYNGTEIKKDTILQKubiquitination[1]
442KKDTILQKGEELPPRubiquitination[1]
476RITAQTSKPLWSVGTubiquitination[1]
553VSDGAINKNPRNKSTubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane. Confers leflunomide resistance when overexpressed. 
Sequence Annotation
 DOMAIN 166 364 B30.2/SPRY.
 MOD_RES 358 358 Phosphoserine.
 CARBOHYD 212 212 N-linked (GlcNAc...) (Potential).
 CARBOHYD 356 356 N-linked (GlcNAc...) (Potential).
 CARBOHYD 430 430 N-linked (GlcNAc...) (Potential).
 CARBOHYD 507 507 N-linked (GlcNAc...) (Potential).
 CARBOHYD 557 557 N-linked (GlcNAc...) (Potential).
 CARBOHYD 575 575 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Endosome; Glycoprotein; Membrane; Phosphoprotein; Protein transport; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Transport; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 579 AA 
Protein Sequence
MYVTFNEALD SSFGNLESPN HDFKVGDPNM VPTPPMDSDS AAISLAFLIS LSITFAILML 60
ILVVIAAYVT FCGDDESEYD EENALGTRTS GTLHSLFGKK HSGILLDSSF ASPGGFDDEI 120
VLQERELEEL PKMSAYEVEL YIRAKEFQMM SPPMVKDFGT YLDSDDQQFI KDRGIQSYFL 180
LPSINDNIDE YGNFLPSFIV QDKLDIQFSK FNKSSSTVMN YPLPHNRKDA VYFEVKIFRH 240
IQKSNSIFSI GLTTVPYPYF RVPGMAKYSI AYESTGKLRI NNPFTASTLL PKLEEGDTVG 300
FGYRYKTGTI FITHNGKKLM DVTQNIGIDL FIGIGAFNAA YTRTYTRDGL LEDPDNVSFR 360
EALSEGKDIE VAKDLQRVHD PHDESDEMTS DEVELHVNLG QVGFVFIEAN VKKYAFGSVY 420
GQIGIPPAYN GTEIKKDTIL QKGEELPPRY ADTDNFFGSM KVKEGSSSRI TAQTSKPLWS 480
VGTYERISSN FDRENNVYHD SLETDDNNTD NNVNNNDENA GCNENSPLLE DDGNKRPENS 540
NTPREVSDGA INKNPRNKST KKRQRNRGKS SKKKNRSRK 579 
Gene Ontology
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0043328; P:protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IGI:SGD.
 GO:0016192; P:vesicle-mediated transport; IGI:SGD. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF00622; SPRY 
SMART
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY 
PRINTS