CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018584
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pre-mRNA-processing factor 6 
Protein Synonyms/Alias
 PRP6 homolog; U5 snRNP-associated 102 kDa protein; U5-102 kDa protein 
Gene Name
 Prpf6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
178QRNPRYEKLTPVPDSubiquitination[1]
334VARNLIMKGTEMCPKubiquitination[1]
606RAVAHCPKAEVLWLMacetylation[2]
727EQGELMEKAREAYNQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Involved in pre-mRNA splicing as component of the U4/U6- U5 tri-snRNP complex, one of the building blocks of the spliceosome (By similarity). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen- induced transactivation (By similarity). 
Sequence Annotation
 REPEAT 384 416 HAT 1.
 REPEAT 418 444 HAT 2.
 REPEAT 445 476 HAT 3.
 REPEAT 554 586 HAT 4.
 REPEAT 588 620 HAT 5.
 REPEAT 622 654 HAT 6.
 REPEAT 689 721 HAT 7.
 REPEAT 723 755 HAT 8.
 REPEAT 855 887 HAT 9.
 MOD_RES 266 266 Phosphothreonine (By similarity).
 MOD_RES 275 275 Phosphothreonine (By similarity).
 MOD_RES 279 279 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 941 AA 
Protein Sequence
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM 60
KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE ADAIYAALDK RMDERRKERR 120
EQREKEEIEK YRMERPKIQQ QFSDLKRKLA EVTEEEWLSI PEVGDARNKR QRNPRYEKLT 180
PVPDSFFAKH LQTGENHTSV DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM 240
RKIGQARNTL MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS 300
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA RLQPGDTAKA 360
VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV PNSVRLWKAA VELEEPEDAR 420
IMLSRAVECC PTSVELWLAL ARLETYENAR KVLNKARENI PTDRHIWITA AKLEEANGNT 480
QMVEKIIDRA ITSLRANGVE INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK 540
HTWMEDADSC VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA 600
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK LESENNEYER 660
ARRLLAKARS SAPTARVFMK SVKLEWVLGN ISAAQELCEE ALRHYEDFPK LWMMKGQIEE 720
QGELMEKARE AYNQGLKKCP HSTPLWLLLS RLEEKIGQLT RARAILEKSR LKNPKNPGLW 780
LESVRLEYRA GLKNIANTLM AKALQECPNS GILWSEAVFL EARPQRKTKS VDALKKCEHD 840
PHVLLAVAKL FWSERKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR 900
CENAEPRHGE LWCAVSKDIT NWQRKIGEIL VLVAARIKNT F 941 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISO:MGI.
 GO:0071001; C:U4/U6 snRNP; IEA:Compara.
 GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IEA:Compara.
 GO:0005682; C:U5 snRNP; IEA:Compara.
 GO:0050681; F:androgen receptor binding; ISO:MGI.
 GO:0043021; F:ribonucleoprotein complex binding; IEA:Compara.
 GO:0003723; F:RNA binding; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; ISO:MGI.
 GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISO:MGI.
 GO:0006403; P:RNA localization; IEA:Compara. 
Interpro
 IPR003107; HAT.
 IPR010491; PRP1_N.
 IPR027108; Prp6/Prp1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF06424; PRP1_N
 PF13181; TPR_8 
SMART
 SM00386; HAT 
PROSITE
  
PRINTS