CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021073
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar protein sorting-associated protein 35 
Protein Synonyms/Alias
 Maternal-embryonic 3; Vesicle protein sorting 35 
Gene Name
 Vps35 
Gene Synonyms/Alias
 Mem3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
14SPQDEQEKLLDEAIQubiquitination[1]
24DEAIQAVKVQSFQMKubiquitination[1]
44NKLMDALKHASNMLGubiquitination[1]
131QSRKDILKDLVEMCRacetylation[2]
239GVNVERYKQIVLTGIacetylation[2]
396ATSSAVSKELTRLLKubiquitination[1]
403KELTRLLKIPVDTYNacetylation[2]
403KELTRLLKIPVDTYNubiquitination[1]
417NNILTVLKLKHFHPLubiquitination[1]
419ILTVLKLKHFHPLFEacetylation[2]
659QCALAASKLLKKPDQacetylation[2]
659QCALAASKLLKKPDQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (By similarity). 
Sequence Annotation
 MOD_RES 7 7 Phosphoserine.
 MOD_RES 791 791 Phosphotyrosine.  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 796 AA 
Protein Sequence
MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML GELRTSMLSP 60
KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY AGNIIPRLYL LITVGVVYVK 120
SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR NYLLQCTRNI LPDEGEPTDE ETTGDISDSM 180
DFVLLNFAEM NKLWVRMQHQ GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ 240
IVLTGILEQV VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII 300
ALIDRLALFA HREDGPGIPA EIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL QVSLINLAMK 360
CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR LLKIPVDTYN NILTVLKLKH 420
FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD 480
PEDFADEQSL VGRFIHLLRS DDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA 540
FRYKENSQMD DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE 600
TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR TQCALAASKL 660
LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL KKALKIANQC MDPSLQVQLF 720
IEILNRYIYF YEKENDAVTI QVLNQLIQKI REDLPNLESS EETEQINKHF HNTLEHLRSR 780
RESPESEGPI YEGLIL 796 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005768; C:endosome; IEA:Compara.
 GO:0016021; C:integral to membrane; ISA:MGI.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006624; P:vacuolar protein processing; ISA:MGI. 
Interpro
 IPR005378; VPS35. 
Pfam
 PF03635; Vps35 
SMART
  
PROSITE
  
PRINTS