CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024550
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-hydroxy-3-methylglutaryl-coenzyme A reductase 
Protein Synonyms/Alias
 HMG-CoA reductase 
Gene Name
 HMGCR 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
248VLEEEENKPNPVTQRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins (By similarity). 
Sequence Annotation
 DOMAIN 61 218 SSD.
 REGION 340 449 Linker.
 REGION 450 888 Catalytic.
 MOTIF 75 78 INSIG-binding motif (By similarity).
 ACT_SITE 559 559 Charge relay system (By similarity).
 ACT_SITE 691 691 Charge relay system (By similarity).
 ACT_SITE 767 767 Charge relay system (By similarity).
 ACT_SITE 866 866 Proton donor (By similarity).
 MOD_RES 872 872 Phosphoserine; by AMPK (By similarity).
 CARBOHYD 281 281 N-linked (GlcNAc...) (Potential).
 CARBOHYD 296 296 N-linked (GlcNAc...) (Potential).
 CARBOHYD 419 419 N-linked (GlcNAc...) (Potential).
 CARBOHYD 518 518 N-linked (GlcNAc...) (Potential).
 CARBOHYD 870 870 N-linked (GlcNAc...) (Potential).
 CROSSLNK 89 89 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 248 248 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Endoplasmic reticulum; Glycoprotein; Isopeptide bond; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 888 AA 
Protein Sequence
MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC PKLEEDVLSS 60
DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI FSSFVFSTVV IHFLDKELTG 120
LNEALPFFLL LVDLSRASAL AKFALSSNSQ DEVRENIARG MAILGPTFTL DALVECLVIG 180
VGTMSGVRQL EIMCCFGCMS VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR 240
VLEEEENKPN PVTQRVKMIM SLGLVLVHAH SRWIADPSPQ NSTADNSKVS LGLDENVSKR 300
IEPSVSLWQF YLSKMISMDI EQVITLSLAL LLAVKYIFFE QAETESTLSL KNPITSPVVT 360
QKKITDDCCR RDPVLVRNDQ KFHAMEEETR KNRERKVEVI KPLLAENDTS HRATFVVGNS 420
SLLGTSLELE TQEPEMELPV EPRPNEECLQ ILENAEKGAK FLSDAEIIQL VNAKHIPAYK 480
LETLMETHER GVSIRRQLLS KKLPEPSSLQ YLPYRDYNYS LVMGACCENV IGYMPIPVGV 540
AGPLCLDGKE FQVPMATTEG CLVASTNRGC RAIGLGGGAS SRVLADGMTR GPVVRFPRAC 600
DSAEVKAWLE TPEGFTVIKE AFDSTSRFAR LQKLHMSVAG RNLYIRFQSR SGDAMGMNMI 660
SKGTEKALSK LQEYFPEMQI LAVSGNYCTD KKPAAINWIE GRGKSVVCEA VIPAKVVREV 720
LKTTTEAMIE VNINKNLVGS AMAGSIGGYN AHAANIVTAI YIACGQDAAQ NVGSSNCITL 780
MEASGPTNED LYISCTMPSI EIGTVGGGTN LLPQQACLQM LGVQGACRDN PGENARQLAR 840
IVCGTVMAGE LSLMAALAAG HLVRSHMIHN RSKINLQDLQ GTCTKKAA 888 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IEA:EC.
 GO:0070402; F:NADPH binding; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
 GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
 GO:0009790; P:embryo development; IEA:Compara.
 GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
 GO:0043407; P:negative regulation of MAP kinase activity; IEA:Compara.
 GO:0051262; P:protein tetramerization; IEA:Compara.
 GO:0008542; P:visual learning; IEA:Compara. 
Interpro
 IPR002202; HMG_CoA_Rdtase.
 IPR023074; HMG_CoA_Rdtase_cat.
 IPR023076; HMG_CoA_Rdtase_CS.
 IPR004554; HMG_CoA_Rdtase_eu_arc.
 IPR004816; HMG_CoA_Rdtase_metazoan.
 IPR023282; HMG_CoA_Rdtase_N.
 IPR009023; HMG_CoA_Rdtase_NAD(P)-bd.
 IPR009029; HMG_CoA_Rdtase_sub-bd.
 IPR000731; SSD. 
Pfam
 PF00368; HMG-CoA_red 
SMART
  
PROSITE
 PS00066; HMG_COA_REDUCTASE_1
 PS00318; HMG_COA_REDUCTASE_2
 PS01192; HMG_COA_REDUCTASE_3
 PS50065; HMG_COA_REDUCTASE_4
 PS50156; SSD 
PRINTS
 PR00071; HMGCOARDTASE.