CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040599
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 BRCA1-A complex subunit RAP80 
Protein Synonyms/Alias
  
Gene Name
 UIMC1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9PRRKKKVKEVSESRNubiquitination[1]
19SESRNLEKKDVETTSubiquitination[2]
20ESRNLEKKDVETTSSubiquitination[1, 2]
31TTSSVSVKRKRRLEDubiquitination[1, 2, 3, 4]
75AKCLAKRKIAQMTEEubiquitination[1, 2]
90EQFALALKMSEQEARubiquitination[1, 2]
112EEEELLRKAIAESLNubiquitination[2]
146SSQSHQEKTTDSGLTubiquitination[2]
166LVPPSLFKGSHISQGubiquitination[2, 5]
188EPWDHTEKTEEEPVSubiquitination[5]
215VFENVNVKSFDRCTGubiquitination[1, 5]
245GRGSAFLKAVQGSGDubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
265LPTLADAKGLQDTGGubiquitination[2, 5, 7]
305CQLEVYQKSLKMAQRubiquitination[5]
345QGEQASEKNECISEDubiquitination[5]
374SDWHSKTKDFQESSIubiquitination[1, 2, 3, 5, 7, 8]
382DFQESSIKSLKEKLLubiquitination[1, 2, 3, 4, 5, 6, 8, 9]
387SIKSLKEKLLLEEEPubiquitination[1, 2, 5, 8]
428SVAALTSKRSLVLMPubiquitination[1, 5]
480VRIIMADKEVGNKEDubiquitination[1, 2]
485ADKEVGNKEDAEKEVubiquitination[1]
545RQKEAKTKSDSGTAAubiquitination[1, 2, 5, 7, 8, 9]
560QTSLDIDKNEKCYLCubiquitination[1, 2, 5]
563LDIDKNEKCYLCKSLubiquitination[2]
568NEKCYLCKSLVPFREubiquitination[2, 5, 7]
588DSCLQLAKADQGDGPubiquitination[1, 2, 5, 8]
608ACSTVEGKWQQRLKNubiquitination[2, 3, 5, 7, 9]
636FLEQSEHKTSDADIKubiquitination[2]
643KTSDADIKSSETGAFubiquitination[1, 2, 5]
697TDCLVDFKKQVTVQPubiquitination[2, 5]
698DCLVDFKKQVTVQPGubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 720 AA 
Protein Sequence
MPRRKKKVKE VSESRNLEKK DVETTSSVSV KRKRRLEDAF IVISDSDGEE PKEENGLQKT 60
KTKQSNRAKC LAKRKIAQMT EEEQFALALK MSEQEAREVN SQEEEEEELL RKAIAESLNS 120
CRPSDASATR SRPLATGPSS QSHQEKTTDS GLTEGIWQLV PPSLFKGSHI SQGNEAEERE 180
EPWDHTEKTE EEPVSGSSGS WDQSSQPVFE NVNVKSFDRC TGHSAEHTQC GKPQESTGRG 240
SAFLKAVQGS GDTSRHCLPT LADAKGLQDT GGTVNYFWGI PFCPDGVDPN QYTKVILCQL 300
EVYQKSLKMA QRQLLNKKGF GEPVLPRPPS LIQNECGQGE QASEKNECIS EDMGDEDKEE 360
RQESRASDWH SKTKDFQESS IKSLKEKLLL EEEPTTSHGQ SSQGIVEETS EEGNSVPASQ 420
SVAALTSKRS LVLMPESSAE EITVCPETQL SSSETFDLER EVSPGSRDIL DGVRIIMADK 480
EVGNKEDAEK EVAISTFSSS NQVSCPLCDQ CFPPTKIERH AMYCNGLMEE DTEVLTRRQK 540
EAKTKSDSGT AAQTSLDIDK NEKCYLCKSL VPFREYQCHV DSCLQLAKAD QGDGPEGSGR 600
ACSTVEGKWQ QRLKNPKEKG HSEGRLLSFL EQSEHKTSDA DIKSSETGAF RVPSPGMEEA 660
GCSREMQSSF TRRDLNESPV KSFVSISEAT DCLVDFKKQV TVQPGSRTRT KAGRGRRRKF 720 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA. 
Interpro
 IPR003903; Ubiquitin-int_motif. 
Pfam
  
SMART
 SM00726; UIM 
PROSITE
 PS50330; UIM 
PRINTS