CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014594
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Meprin A subunit alpha 
Protein Synonyms/Alias
 Endopeptidase-2; Endopeptidase-24.18 subunit alpha; E-24.18; MEP-1 
Gene Name
 Mep1a 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
377GNVCQLAKIQTFQGDacetylation[1]
400HVTLNEEKKFRYVFQacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 DOMAIN 265 434 MAM.
 DOMAIN 435 596 MATH.
 DOMAIN 672 712 EGF-like.
 REGION 67 264 Metalloprotease.
 ACT_SITE 157 157 By similarity.
 METAL 156 156 Zinc; catalytic (By similarity).
 METAL 160 160 Zinc; catalytic (By similarity).
 METAL 166 166 Zinc; catalytic (By similarity).
 CARBOHYD 141 141 N-linked (GlcNAc...) (Potential).
 CARBOHYD 223 223 N-linked (GlcNAc...) (Potential).
 CARBOHYD 259 259 N-linked (GlcNAc...) (Potential).
 CARBOHYD 319 319 N-linked (GlcNAc...) (Potential).
 CARBOHYD 441 441 N-linked (GlcNAc...) (Potential).
 CARBOHYD 542 542 N-linked (GlcNAc...) (Potential).
 DISULFID 108 260 By similarity.
 DISULFID 129 148 By similarity.
 DISULFID 270 432 By similarity.
 DISULFID 278 278 Interchain (By similarity).
 DISULFID 309 309 Interchain.
 DISULFID 676 687 By similarity.
 DISULFID 681 696 By similarity.
 DISULFID 698 711 By similarity.  
Keyword
 Complete proteome; Disulfide bond; EGF-like domain; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Protease; Reference proteome; Signal; Transmembrane; Transmembrane helix; Zinc; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 748 AA 
Protein Sequence
MLWTLPVCLL SLSFSAHIAA VSIQHLSTGH DHDDVDVGEQ QKDISEINSA AGLNLFQGDI 60
LLPRTRNALR DPSSRWKLPI PYILADNLDL NAKGAILNAF EMFRLKSCVD FKPYEGESSY 120
IIFQQFSGCW SMVGDQHVGQ NISIGEGCDY KAIIEHEILH ALGFFHEQSR TDRDDYVNIW 180
WNEIMTDYEH NFNTYDDKTI TDLNTPYDYE SLMHYGPFSF NKNETIPTIT TKIPEFNAII 240
GQRLDFSATD LTRLNRMYNC TRTHTLLDHC AFEKTNICGM IQGTRDDADW VHEDSSQPGQ 300
VDHTLVGRCK AAGYFMYFNT SSGVTGEVAL LESRILYPKR KQQCLQFFYK MTGSPADRLL 360
IWVRRDDNTG NVCQLAKIQT FQGDSDHNWK IAHVTLNEEK KFRYVFQGTK GDPGNSDGGI 420
YLDDITLTET PCPTGVWTIR NISQVLENTV KGDRLVSPRF YNSEGYGFGV TLYPNGRITS 480
NSGYLGLAFH LYSGDNDVIL EWPVENRQAI MTILDQEPDA RNRMSLSLMF TTSKYQTSSA 540
INGSVIWDRP TKVGVYDKDC DCFRSIDWGW GQAISHQMLM RRNFLKDDTL IIFVDFKDLT 600
HLRQTEVPIP SRSVIPRGLL LQGQEPLALG DSRIAMMEES LPRRLDQRQP SRPKRSVENT 660
GPMEDHNWPQ YFRDPCDPNP CQNEGTCVNV KGMASCRCVS GHAFFYTGER CQAMHVHGSL 720
LGLLIGCITA LIFLTFITFS NTYQKLRQ 748 
Gene Ontology
 GO:0017090; C:meprin A complex; IDA:RGD.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006508; P:proteolysis; NAS:RGD. 
Interpro
 IPR008985; ConA-like_lec_gl_sf.
 IPR000742; EG-like_dom.
 IPR000998; MAM_dom.
 IPR002083; MATH.
 IPR024079; MetalloPept_cat_dom.
 IPR008294; Pept_M12A_Meprin.
 IPR001506; Peptidase_M12A.
 IPR006026; Peptidase_Metallo.
 IPR008974; TRAF-like. 
Pfam
 PF01400; Astacin
 PF00008; EGF
 PF00629; MAM
 PF00917; MATH 
SMART
 SM00181; EGF
 SM00137; MAM
 SM00061; MATH
 SM00235; ZnMc 
PROSITE
 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3
 PS00740; MAM_1
 PS50060; MAM_2
 PS50144; MATH
 PS00142; ZINC_PROTEASE 
PRINTS
 PR00480; ASTACIN.
 PR00020; MAMDOMAIN.