CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000481
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intermediate conductance calcium-activated potassium channel protein 4 
Protein Synonyms/Alias
 SK4; SKCa 4; SKCa4; IKCa1; IK1; KCa3.1; KCa4; Putative Gardos channel 
Gene Name
 KCNN4 
Gene Synonyms/Alias
 IK1; IKCA1; KCA4; SK4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
394SSHRALEKQIDTLAGubiquitination[1, 2, 3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. 
Sequence Annotation
 REGION 286 347 Calmodulin-binding.
 MOD_RES 358 358 Phosphohistidine.  
Keyword
 Calmodulin-binding; Complete proteome; Immunity; Ion channel; Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 427 AA 
Protein Sequence
MGGDLVLGLG ALRRRKRLLE QEKSLAGWAL VLAGTGIGLM VLHAEMLWFG GCSWALYLFL 60
VKCTISISTF LLLCLIVAFH AKEVQLFMTD NGLRDWRVAL TGRQAAQIVL ELVVCGLHPA 120
PVRGPPCVQD LGAPLTSPQP WPGFLGQGEA LLSLAMLLRL YLVPRAVLLR SGVLLNASYR 180
SIGALNQVRF RHWFVAKLYM NTHPGRLLLG LTLGLWLTTA WVLSVAERQA VNATGHLSDT 240
LWLIPITFLT IGYGDVVPGT MWGKIVCLCT GVMGVCCTAL LVAVVARKLE FNKAEKHVHN 300
FMMDIQYTKE MKESAARVLQ EAWMFYKHTR RKESHAARRH QRKLLAAINA FRQVRLKHRK 360
LREQVNSMVD ISKMHMILYD LQQNLSSSHR ALEKQIDTLA GKLDALTELL STALGPRQLP 420
EPSQQSK 427 
Gene Ontology
 GO:0008076; C:voltage-gated potassium channel complex; IC:BHF-UCL.
 GO:0015269; F:calcium-activated potassium channel activity; IDA:BHF-UCL.
 GO:0016286; F:small conductance calcium-activated potassium channel activity; IEA:InterPro.
 GO:0006816; P:calcium ion transport; IDA:BHF-UCL.
 GO:0006884; P:cell volume homeostasis; IEA:Compara.
 GO:0006952; P:defense response; TAS:ProtInc.
 GO:0045332; P:phospholipid translocation; IEA:Compara.
 GO:0050714; P:positive regulation of protein secretion; IEA:Compara.
 GO:0050862; P:positive regulation of T cell receptor signaling pathway; IDA:BHF-UCL.
 GO:0046541; P:saliva secretion; IEA:Compara.
 GO:0030322; P:stabilization of membrane potential; IDA:BHF-UCL.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 
Interpro
 IPR004178; CaM-bd_dom.
 IPR013099; Ion_trans_2.
 IPR015449; K_chnl_Ca-activ_SK. 
Pfam
 PF02888; CaMBD
 PF07885; Ion_trans_2
 PF03530; SK_channel 
SMART
 SM01053; CaMBD 
PROSITE
  
PRINTS