UniProt Accession

 PSA2_YEAST; P23639; D6W0J3 

Genbank Protein ID

 X56731; Z46660; AY557762; BK006946 

Genbank Nucleotide ID

 CAA40055.1; CAA86646.1; AAS56088.1; DAA09807.1 

Protein Name

 Proteasome subunit alpha type-2 

Protein Synonyms/Alias

 Macropain subunit Y7; Multicatalytic endopeptidase complex subunit Y7; Proteasome component Y7; Proteinase YSCE subunit 7 

Gene Name

 PRE8 

Gene Synonyms/Alias

 PRS4; YML092C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
29	DYALTAVKQGVTSLG	ubiquitination	[1, 2]
88	DYRVLVDKSRKVAHT	ubiquitination	[2]
108	YGEYPPTKLLVSEVA	ubiquitination	[1, 2]
166	WKATAIGKGSVAAKT	ubiquitination	[2]
172	GKGSVAAKTFLEKRW	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleus; Protease; Proteasome; Reference proteome; Threonine protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 250 AA 

Protein Sequence

Gene Ontology

 GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; IC:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0019773; C:proteasome core complex, alpha-subunit complex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:SGD. 

Interpro

 IPR000426; Proteasome_asu_N.
 IPR023332; Proteasome_suA-type.
 IPR001353; Proteasome_sua/b. 

Pfam

 PF00227; Proteasome
 PF10584; Proteasome_A_N 

SMART

 SM00948; Proteasome_A_N 

PROSITE

 PS00388; PROTEASOME_A_1
 PS51475; PROTEASOME_A_2 

PRINTS