CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011350
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA-dihydrouridine(47) synthase [NAD(P)(+)] 
Protein Synonyms/Alias
 tRNA-dihydrouridine synthase 3 
Gene Name
 DUS3 
Gene Synonyms/Alias
 YLR401C; L8084.19 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
173DNILISKKEIDPDAQacetylation[1]
229QECRDSMKPEEEVESacetylation[1]
242ESTPQLKKQDPDVEQubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. 
Sequence Annotation
 ZN_FING 92 122 C3H1-type 1.
 ZN_FING 134 164 C3H1-type 2.  
Keyword
 Complete proteome; Cytoplasm; FAD; Flavoprotein; Metal-binding; NAD; NADP; Nucleus; Oxidoreductase; Reference proteome; Repeat; tRNA processing; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 668 AA 
Protein Sequence
MEQNAEKRSI VGDDNSTVKR QDTSPSKGIA HIKPEYIVPL KQNENQKVAI YDEEMSSDRM 60
TNEFAGGTNK KNKNGRGKKR GQNKNRDNRQ VKEQNVLCPR LIHGDISKCS FGDNCRFVHD 120
INLYLSTKKP EVESNIFPSC PVFNSLGFCP MGFKCRFLSS HLNKEDNILI SKKEIDPDAQ 180
TIWSVKGEVN HISPERKLDL IKRRFPFTKS NEILEIIDSF QQECRDSMKP EEEVESTPQL 240
KKQDPDVEQP VAPQVEQRNK ELSEHRMKQR EVYLKYKDTR YFAQEKKPLD LYHKKIVSPL 300
TTVGNLPYRR LMRKLGADVT YSEMALAVPL IQGTNSEWAL PKAHTSEFPG FGVQVACSKA 360
WQAAKAAEAL ANSVSEISEI NLNSGCPIDL LYRQGSGSAL LDNPARMIRC LNAMNYVSKD 420
IPITVKIRTG TKEGHPIAEG LVKRLVNETD VAAITLHGRS RQQRYTKSAD WDYVSQVADT 480
LRSAEADFIE TEQGKEGRDS KNRIQFVGNG DVNNFEDWYR YLNGNENIDS VMVARGALIK 540
PWIFEEVESQ QYLDKTSTER LDILRDYAQF SMEHWGTDEY GISQCRRFFC EFMSFFHRYV 600
PMGICERYPV KLNERPPNWC GRDELETLMG STDVNDWIKL SDLFFGKTDE NFVFVPKHKS 660
SSYANRDS 668 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0017150; F:tRNA dihydrouridine synthase activity; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR001269; tRNA_hU_synthase.
 IPR018517; tRNA_hU_synthase_CS.
 IPR000571; Znf_CCCH. 
Pfam
 PF01207; Dus 
SMART
  
PROSITE
 PS01136; UPF0034
 PS50103; ZF_C3H1 
PRINTS