CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018062
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U3 small nucleolar RNA-associated protein 15 homolog 
Protein Synonyms/Alias
  
Gene Name
 UTP15 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MAGYKPVAIQTYubiquitination[1]
30TLYWNNYKTPVQIKEubiquitination[2]
36YKTPVQIKEFGAVSKubiquitination[1, 2, 3, 4, 5]
80IKTFSRFKDTAYCATubiquitination[1, 6]
155LWDIPNSKEILTFKEubiquitination[1, 2, 3, 4]
161SKEILTFKEHSDYVRubiquitination[1]
190GSYDHTVKMFDARTSubiquitination[2]
359LINRPAKKHLELYDRubiquitination[1]
369ELYDRDLKHFRISKAubiquitination[1]
375LKHFRISKALDRVLDubiquitination[1]
398EITVSIIKELNRRGVubiquitination[1, 3]
472LLQGLVEKEIDYQREubiquitination[4]
497LFATMRRKEGTSVLEubiquitination[4]
514SDGFPENKKIES***ubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Involved in nucleolar processing of pre-18S ribosomal RNA (By similarity). 
Sequence Annotation
 REPEAT 36 75 WD 1.
 REPEAT 78 117 WD 2.
 REPEAT 120 159 WD 3.
 REPEAT 162 202 WD 4.
 REPEAT 204 242 WD 5.
 REPEAT 246 285 WD 6.
 REPEAT 287 326 WD 7.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Complete proteome; Nucleus; Polymorphism; Reference proteome; Repeat; rRNA processing; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 518 AA 
Protein Sequence
MAGYKPVAIQ TYPILGEKIT QDTLYWNNYK TPVQIKEFGA VSKVDFSPQP PYNYAVTASS 60
RIHIYGRYSQ EPIKTFSRFK DTAYCATFRQ DGRLLVAGSE DGGVQLFDIS GRAPLRQFEG 120
HTKAVHTVDF TADKYHVVSG ADDYTVKLWD IPNSKEILTF KEHSDYVRCG CASKLNPDLF 180
ITGSYDHTVK MFDARTSESV LSVEHGQPVE SVLLFPSGGL LVSAGGRYVK VWDMLKGGQL 240
LVSLKNHHKT VTCLCLSSSG QRLLSGSLDR KVKVYSTTSY KVVHSFDYAA SILSLALAHE 300
DETIVVGMTN GILSVKHRKS EAKKESLPRR RRPAYRTFIK GKNYMKQRDD ILINRPAKKH 360
LELYDRDLKH FRISKALDRV LDPTCTIKTP EITVSIIKEL NRRGVLANAL AGRDEKEISH 420
VLNFLIRNLS QPRFAPVLIN AAEIIIDIYL PVIGQSPVVD KKFLLLQGLV EKEIDYQREL 480
LETLGMMDML FATMRRKEGT SVLEHTSDGF PENKKIES 518 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:LIFEdb.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR018983; U3_snoRNA-assocProt_15_C.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF09384; UTP15_C
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS