CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000849
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit beta' 
Protein Synonyms/Alias
 Beta'-coat protein; Beta'-COP; p102 
Gene Name
 Copb2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
64DLPVRAAKFVARKNWacetylation[1]
212GADDRLVKIWDYQNKacetylation[1]
212GADDRLVKIWDYQNKubiquitination[2]
318NGKIIWAKHSEVQQAacetylation[1]
318NGKIIWAKHSEVQQAubiquitination[2]
328EVQQANLKAMGDTEIubiquitination[2]
386TAMALRNKSFGSAQEacetylation[3]
569YLLGYIPKDNRLYLGacetylation[1]
608RDFSMADKVLPTIPKubiquitination[2]
627RVAHFLEKQGFKQQAacetylation[1]
627RVAHFLEKQGFKQQAubiquitination[2]
783NLSKVNQKAAESLADubiquitination[2]
822HADLWPAKQYPLVTPubiquitination[2]
871MASQTTHKEEKSLLEubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). 
Sequence Annotation
 REPEAT 13 52 WD 1.
 REPEAT 55 94 WD 2.
 REPEAT 97 136 WD 3.
 REPEAT 140 180 WD 4.
 REPEAT 183 224 WD 5.
 REPEAT 227 266 WD 6.
 REPEAT 350 388 WD 7.
 REPEAT 390 425 WD 8.
 REPEAT 746 783 WD 9.
 MOD_RES 627 627 N6-acetyllysine (By similarity).
 MOD_RES 859 859 Phosphoserine (By similarity).  
Keyword
 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 905 AA 
Protein Sequence
MPLRLDIKRK LTARSDRVKS VDLHPTEPWM LASLYNGSVC VWNHETQTLV KTFEVCDLPV 60
RAAKFVARKN WVVTGADDMQ IRVFNYNTLE RVHMFEAHSD YIRCIAVHPT QPFILTSSDD 120
MLIKLWDWDK KWSCSQVFEG HTHYVMQIVI NPKDNNQFAS ASLDRTIKVW QLGSSSPNFT 180
LEGHEKGVNC IDYYSGGDKP YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NVSCASFHPE 240
LPIIITGSED GTVRIWHSST YRLESTLNYG MERVWCVASL RGSNNVALGY DEGSIIVKLG 300
REEPAMSMDA NGKIIWAKHS EVQQANLKAM GDTEIKDGER LPLAVKDMGS CEIYPQTIQH 360
NPNGRFVVVC GDGEYIIYTA MALRNKSFGS AQEFAWAHDS SEYAIRESNS IVKIFKNFKE 420
KKSFKPDFGA ESIYGGFLLG VRSVNGLAFY DWENTELIRR IEIQPKHIFW SDSGELVCIA 480
TEESFFILKY LSEKVLAAQE THEGVTEDGI EDAFEVLGEI QEIVKTGLWV GDCFIYTSSV 540
NRLNYYVGGE IVTIAHLDRT MYLLGYIPKD NRLYLGDKEL NIVSYSLLVS VLEYQTAVMR 600
RDFSMADKVL PTIPKEQRTR VAHFLEKQGF KQQALTVSTD PEHRFELALQ LGELKIAYQL 660
AVEAESEQKW KQLAELAISK CQFSLAQECL HHAQDYGGLL LLATASGNAS MVNKLAEGAE 720
RDGKNNVAFM SYFLQGKLDA CLELLIRTGR LPEAAFLART YLPSQVSRVV KLWRENLSKV 780
NQKAAESLAD PTEYENLFPG LKEAFVVEEW VKETHADLWP AKQYPLVTPN EERNVMEEAK 840
GFQPSRPTAQ QEPDGKPASS PVIMASQTTH KEEKSLLELE VDLDNLELED IDTTDINLDE 900
DILDD 905 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0030126; C:COPI vesicle coat; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IDA:MGI.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; IEA:Compara.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR006692; Coatomer_WD-assoc_reg.
 IPR016453; COPB2.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04053; Coatomer_WDAD
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.