CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008838
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Arginine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Arginyl-tRNA synthetase; ArgRS 
Gene Name
 RARS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
321TSDLAAIKQRLFEEKubiquitination[1]
399DLLGEGLKRSMDKLKubiquitination[1]
485IDEEMLQKAARETKIubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1. 
Sequence Annotation
 REGION 1 72 Could be involved in the assembly of the
 MOTIF 201 212 "HIGH" region.
 MOD_RES 1 1 N-acetylmethionine.  
Keyword
 Acetylation; Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Polymorphism; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 660 AA 
Protein Sequence
MINIISRLQE VFGHAIKAAY PDLENPPLLV TPSQQAKFGD YQCNSAMGIS QMLKTKEQKV 60
NPREIAENIT KHLPDNECIE KVEIAGPGFI NVHLRKDFVS EQLTSLLVNG VQLPALGENK 120
KVIVDFSSPN IAKEMHVGHL RSTIIGESIS RLFEFAGYDV LRLNHVGDWG TQFGMLIAHL 180
QDKFPDYLTV SPPIGDLQVF YKESKKRFDT EEEFKKRAYQ CVVLLQGKNP DITKAWKLIC 240
DVSRQELNKI YDALDVSLIE RGESFYQDRM NDIVKEFEDR GFVQVDDGRK IVFVPGCSIP 300
LTIVKSDGGY TYDTSDLAAI KQRLFEEKAD MIIYVVDNGQ SVHFQTIFAA AQMIGWYDPK 360
VTRVFHAGFG VVLGEDKKKF KTRSGETVRL MDLLGEGLKR SMDKLKEKER DKVLTAEELN 420
AAQTSVAYGC IKYADLSHNR LNDYIFSFDK MLDDRGNTAA YLLYAFTRIR SIARLANIDE 480
EMLQKAARET KILLDHEKEW KLGRCILRFP EILQKILDDL FLHTLCDYIY ELATAFTEFY 540
DSCYCVEKDR QTGKILKVNM WRMLLCEAVA AVMAKGFDIL GIKPVQRM 588 
Gene Ontology
 GO:0017101; C:aminoacyl-tRNA synthetase multienzyme complex; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0034618; F:arginine binding; IEA:Compara.
 GO:0004814; F:arginine-tRNA ligase activity; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000049; F:tRNA binding; IEA:Compara.
 GO:0006420; P:arginyl-tRNA aminoacylation; TAS:ProtInc. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR001278; Arg-tRNA-ligase_Ia.
 IPR015945; Arg-tRNA-synth_Ia_core.
 IPR005148; Arg-tRNA-synth_N.
 IPR008909; DALR_anticod-bd.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF03485; Arg_tRNA_synt_N
 PF05746; DALR_1
 PF00750; tRNA-synt_1d 
SMART
 SM01016; Arg_tRNA_synt_N
 SM00836; DALR_1 
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR01038; TRNASYNTHARG.