UniProt Accession

 SNF8_HUMAN; Q96H20; Q8IXY3; Q9UN50 

Genbank Protein ID

 AF156102; BC008976; BC038830 

Genbank Nucleotide ID

 AAD46560.1; AAH08976.1; AAH38830.1 

Protein Name

 Vacuolar-sorting protein SNF8 

Protein Synonyms/Alias

 ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22 

Gene Name

 SNF8 

Gene Synonyms/Alias

 EAP30 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
14	GAGAIAKKKLAEAKY	ubiquitination	[1]
38	DQLAQMSKQLDMFKT	ubiquitination	[2, 3, 4, 5]
44	SKQLDMFKTNLEEFA	ubiquitination	[1, 3]
53	NLEEFASKHKQEIRK	ubiquitination	[1, 3]
130	ELHQQVLKGRGKFAQ	ubiquitination	[1, 3]
134	QVLKGRGKFAQDVSQ	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
203	SEIKASLKWETERAR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. 

Sequence Annotation

  

Keyword

 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Endosome; Membrane; Nucleus; Protein transport; Reference proteome; Transcription; Transcription regulation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 258 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0008134; F:transcription factor binding; IDA:MGI.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0016197; P:endosomal transport; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR007286; EAP30.
 IPR016689; ESCRT-2_cplx_Snf8.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF04157; EAP30 

SMART

  

PROSITE

  

PRINTS