| Tag | Content |
|---|
| CPLM ID | CPLM-002710 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Phosphoenolpyruvate-protein phosphotransferase |
Protein Synonyms/Alias | Phosphotransferase system, enzyme I |
Gene Name | ptsI |
Gene Synonyms/Alias | BSU13910 |
Created Date | July 27, 2013 |
Organism | Bacillus subtilis (strain 168) |
NCBI Taxa ID | 224308 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 566 | EEVVAFVKETFK*** | acetylation | [1] |
|
Reference | [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis. Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG. Proteomics. 2013 May;13(10-11):1726-36. [ PMID: 23468065] |
Functional Description | General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr). |
Sequence Annotation | ACT_SITE 189 189 Tele-phosphohistidine intermediate (By
ACT_SITE 502 502 Proton donor (By similarity).
METAL 431 431 Magnesium (By similarity).
METAL 455 455 Magnesium (By similarity).
BINDING 296 296 Substrate (By similarity).
BINDING 332 332 Substrate (By similarity).
BINDING 431 431 Substrate (By similarity).
BINDING 452 452 Substrate; via carbonyl oxygen (By
BINDING 453 453 Substrate; via amide nitrogen (By
BINDING 454 454 Substrate (By similarity).
BINDING 455 455 Substrate; via amide nitrogen (By |
Keyword | Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 570 AA |
Protein Sequence | MQELKGIGAS AGIAIAKAYR LEEPDLTVEK KNISDSEAEV SRFDEAIARS KEELEKIKEH 60
ALKELGQDKA DIFSAHLLVL SDPELLNPVK EKISTDSVNA EFALKETSSM FVTMFESMDN 120
EYMKERAADI RDVTKRVTGH LLGVEIPNPS MISEEVIIVA EDLTPSDTAQ LNREFVKGFT 180
TDIGGRTSHS AIMARSLEIP AVVGTKAATG TIQNGVTVIV DGINGDVIID PSAETVKEYE 240
EKHNAYLAQK AEWAKLVNEP TVSKDGHHVE LAANIGTPDD VKGVLENGGE AVGLYRTEFL 300
YMGRDQLPTE DEQFDAYKTV LERMEGKSVV VRTLDIGGDK ELPYLQLPKE MNPFLGYRAI 360
RLCLEEQEIF RTQLRALLRA STYGNLKIMF PMIATVNEFK EAKAILLEEK EKLVKAGQAV 420
SDDIEVGMMV EIPSTAVIAD QFAKEVDFFS IGTNDLIQYT MAADRMNERV SYLYQPYNPA 480
ILRLITLVIE AAHKEGKWVG MCGEMAGDEI AIPILLGLGL DEFSMSATSI LPARTQISKL 540
SKQEAESFKE KILSMSTTEE VVAFVKETFK 570 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0016301; F:kinase activity; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:EC. GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro. GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |