CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003850
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 L-gulonolactone oxidase 
Protein Synonyms/Alias
 LGO; L-gulono-gamma-lactone oxidase; GLO 
Gene Name
 Gulo 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
6**MVHGYKGVQFQNWacetylation[1]
80NRVLQVDKEKKQITVacetylation[1]
211DNLDSHLKRSEYFRFacetylation[1]
423KFCDIREKLDPTGMFacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L- ascorbate. 
Sequence Annotation
 DOMAIN 17 187 FAD-binding PCMH-type.
 MOD_RES 54 54 Pros-8alpha-FAD histidine (By  
Keyword
 Ascorbate biosynthesis; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; FAD; Flavoprotein; Membrane; Microsome; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 440 AA 
Protein Sequence
MVHGYKGVQF QNWAKTYGCS PEVYYQPTSV EEVREVLALA REQKKKVKVV GGGHSPSDIA 60
CTDGFMIHMG KMNRVLQVDK EKKQITVEAG ILLADLHPQL DEHGLAMSNL GAVSDVTVAG 120
VIGSGTHNTG IKHGILATQV VALTLMTADG EVLECSESRN ADVFQAARVH LGCLGIILTV 180
TLQCVPQFHL QETSFPSTLK EVLDNLDSHL KRSEYFRFLW FPHTENVSII YQDHTNKAPS 240
SASNWFWDYA IGFYLLEFLL WTSTYLPCLV GWINRFFFWM LFNCKKESSN LSHKIFTYEC 300
RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRGDDIL LSPCFQRDSC 360
YMNIIMYRPY GKDVPRLDYW LAYETIMKKF GGRPHWAKAH NCTQKDFEEM YPTFHKFCDI 420
REKLDPTGMF LNSYLEKVFY 440 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0003885; F:D-arabinono-1,4-lactone oxidase activity; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; ISS:UniProtKB.
 GO:0050105; F:L-gulonolactone oxidase activity; ISS:UniProtKB.
 GO:0008762; F:UDP-N-acetylmuramate dehydrogenase activity; IEA:InterPro.
 GO:0019853; P:L-ascorbic acid biosynthetic process; ISS:UniProtKB. 
Interpro
 IPR007173; ALO.
 IPR016169; CO_DH_flavot_FAD-bd_sub2.
 IPR016166; FAD-bd_2.
 IPR016167; FAD-bd_2_sub1.
 IPR010031; FAD_lactone_oxidase.
 IPR010032; FAD_linked_OxRdtase_bac.
 IPR023595; LGO_GLO.
 IPR006094; Oxid_FAD_bind_N.
 IPR006093; Oxy_OxRdtase_FAD_BS. 
Pfam
 PF04030; ALO
 PF01565; FAD_binding_4 
SMART
  
PROSITE
 PS51387; FAD_PCMH
 PS00862; OX2_COVAL_FAD 
PRINTS