CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006676
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Receptor-type tyrosine-protein kinase FLT3 
Protein Synonyms/Alias
 FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD135 
Gene Name
 FLT3 
Gene Synonyms/Alias
 CD135; FLK2; STK1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
614RENLEFGKVLGSGAFubiquitination[1]
623LGSGAFGKVMNATAYubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways. 
Sequence Annotation
 DOMAIN 253 343 Ig-like C2-type.
 DOMAIN 610 943 Protein kinase.
 NP_BIND 616 624 ATP (By similarity).
 REGION 591 597 Important for normal regulation of the
 ACT_SITE 811 811 Proton acceptor (By similarity).
 BINDING 644 644 ATP (Probable).
 MOD_RES 572 572 Phosphotyrosine.
 MOD_RES 574 574 Phosphoserine.
 MOD_RES 589 589 Phosphotyrosine; by autocatalysis.
 MOD_RES 591 591 Phosphotyrosine; by autocatalysis.
 MOD_RES 599 599 Phosphotyrosine; by autocatalysis.
 MOD_RES 726 726 Phosphotyrosine; by autocatalysis.
 MOD_RES 759 759 Phosphoserine.
 MOD_RES 768 768 Phosphotyrosine.
 MOD_RES 793 793 Phosphotyrosine.
 MOD_RES 842 842 Phosphotyrosine; by autocatalysis.
 MOD_RES 955 955 Phosphotyrosine; by autocatalysis.
 MOD_RES 969 969 Phosphotyrosine; by autocatalysis.
 MOD_RES 993 993 Phosphoserine.
 CARBOHYD 43 43 N-linked (GlcNAc...).
 CARBOHYD 100 100 N-linked (GlcNAc...).
 CARBOHYD 151 151 N-linked (GlcNAc...).
 CARBOHYD 306 306 N-linked (GlcNAc...).
 CARBOHYD 323 323 N-linked (GlcNAc...).
 CARBOHYD 351 351 N-linked (GlcNAc...).
 CARBOHYD 354 354 N-linked (GlcNAc...).
 CARBOHYD 473 473 N-linked (GlcNAc...).
 CARBOHYD 502 502 N-linked (GlcNAc...).
 CARBOHYD 541 541 N-linked (GlcNAc...) (Potential).
 DISULFID 35 65
 DISULFID 103 114
 DISULFID 199 206
 DISULFID 232 241
 DISULFID 272 330
 DISULFID 368 407
 DISULFID 381 392  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Immunoglobulin domain; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Receptor; Reference proteome; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 993 AA 
Protein Sequence
MPALARDGGQ LPLLVVFSAM IFGTITNQDL PVIKCVLINH KNNDSSVGKS SSYPMVSESP 60
EDLGCALRPQ SSGTVYEAAA VEVDVSASIT LQVLVDAPGN ISCLWVFKHS SLNCQPHFDL 120
QNRGVVSMVI LKMTETQAGE YLLFIQSEAT NYTILFTVSI RNTLLYTLRR PYFRKMENQD 180
ALVCISESVP EPIVEWVLCD SQGESCKEES PAVVKKEEKV LHELFGTDIR CCARNELGRE 240
CTRLFTIDLN QTPQTTLPQL FLKVGEPLWI RCKAVHVNHG FGLTWELENK ALEEGNYFEM 300
STYSTNRTMI RILFAFVSSV ARNDTGYYTC SSSKHPSQSA LVTIVEKGFI NATNSSEDYE 360
IDQYEEFCFS VRFKAYPQIR CTWTFSRKSF PCEQKGLDNG YSISKFCNHK HQPGEYIFHA 420
ENDDAQFTKM FTLNIRRKPQ VLAEASASQA SCFSDGYPLP SWTWKKCSDK SPNCTEEITE 480
GVWNRKANRK VFGQWVSSST LNMSEAIKGF LVKCCAYNSL GTSCETILLN SPGPFPFIQD 540
NISFYATIGV CLLFIVVLTL LICHKYKKQF RYESQLQMVQ VTGSSDNEYF YVDFREYEYD 600
LKWEFPRENL EFGKVLGSGA FGKVMNATAY GISKTGVSIQ VAVKMLKEKA DSSEREALMS 660
ELKMMTQLGS HENIVNLLGA CTLSGPIYLI FEYCCYGDLL NYLRSKREKF HRTWTEIFKE 720
HNFSFYPTFQ SHPNSSMPGS REVQIHPDSD QISGLHGNSF HSEDEIEYEN QKRLEEEEDL 780
NVLTFEDLLC FAYQVAKGME FLEFKSCVHR DLAARNVLVT HGKVVKICDF GLARDIMSDS 840
NYVVRGNARL PVKWMAPESL FEGIYTIKSD VWSYGILLWE IFSLGVNPYP GIPVDANFYK 900
LIQNGFKMDQ PFYATEEIYI IMQSCWAFDS RKRPSFPNLT SFLGCQLADA EEAMYQNVDG 960
RVSECPHTYQ NRRPFSREMD LGLLSPQAQV EDS 993 
Gene Ontology
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004896; F:cytokine receptor activity; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; TAS:UniProtKB.
 GO:0005021; F:vascular endothelial growth factor-activated receptor activity; TAS:ProtInc.
 GO:0030183; P:B cell differentiation; ISS:UniProtKB.
 GO:0035726; P:common myeloid progenitor cell proliferation; ISS:UniProtKB.
 GO:0097028; P:dendritic cell differentiation; ISS:UniProtKB.
 GO:0001776; P:leukocyte homeostasis; ISS:UniProtKB.
 GO:0046651; P:lymphocyte proliferation; ISS:UniProtKB.
 GO:0002318; P:myeloid progenitor cell differentiation; ISS:UniProtKB.
 GO:0045578; P:negative regulation of B cell differentiation; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; TAS:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; TAS:UniProtKB.
 GO:0043406; P:positive regulation of MAP kinase activity; TAS:UniProtKB.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; TAS:UniProtKB.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; TAS:UniProtKB.
 GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; TAS:UniProtKB.
 GO:0002328; P:pro-B cell differentiation; ISS:UniProtKB.
 GO:0002572; P:pro-T cell differentiation; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; TAS:UniProtKB.
 GO:0042981; P:regulation of apoptotic process; TAS:UniProtKB. 
Interpro
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR013151; Immunoglobulin.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom.
 IPR016243; Tyr_kinase_CSF1/PDGF_rcpt.
 IPR001824; Tyr_kinase_rcpt_3_CS. 
Pfam
 PF00047; ig
 PF07714; Pkinase_Tyr 
SMART
 SM00409; IG
 SM00219; TyrKc 
PROSITE
 PS50835; IG_LIKE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS00240; RECEPTOR_TYR_KIN_III 
PRINTS