CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005838
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutathione S-transferase A3 
Protein Synonyms/Alias
 GST class-alpha member 3; Glutathione S-transferase Ya3; Glutathione S-transferase Yc 
Gene Name
 Gsta3 
Gene Synonyms/Alias
 Gstyc 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
4****MAGKPVLHYFDacetylation[1, 2, 3, 4]
4****MAGKPVLHYFDsuccinylation[4]
4****MAGKPVLHYFDubiquitination[5]
64MVEIDGMKLVQTKAIacetylation[2]
64MVEIDGMKLVQTKAIubiquitination[5]
69GMKLVQTKAILNYIAubiquitination[5]
78ILNYIASKYNLYGKDacetylation[1, 2]
78ILNYIASKYNLYGKDubiquitination[5]
84SKYNLYGKDMKERAIacetylation[2, 3, 6, 7]
84SKYNLYGKDMKERAIubiquitination[5]
123EKEASLAKIKEQTRNacetylation[7]
138RYFPAFEKVLKSHGQacetylation[1, 3, 4, 7]
138RYFPAFEKVLKSHGQsuccinylation[4]
138RYFPAFEKVLKSHGQubiquitination[5]
141PAFEKVLKSHGQDYLacetylation[3]
141PAFEKVLKSHGQDYLubiquitination[5]
195VSNLPTVKKFLQPGSacetylation[2, 4, 7, 8]
195VSNLPTVKKFLQPGSsuccinylation[4]
195VSNLPTVKKFLQPGSubiquitination[5]
196SNLPTVKKFLQPGSQacetylation[2]
196SNLPTVKKFLQPGSQubiquitination[5]
211RKPFDDAKCVESAKKacetylation[2]
211RKPFDDAKCVESAKKubiquitination[5]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753
Functional Description
 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide. 
Sequence Annotation
 DOMAIN 3 83 GST N-terminal.
 DOMAIN 85 207 GST C-terminal.
 REGION 54 55 Glutathione binding (By similarity).
 REGION 67 68 Glutathione binding (By similarity).
 BINDING 9 9 Glutathione (By similarity).
 BINDING 45 45 Glutathione (By similarity).
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 221 AA 
Protein Sequence
MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL MFQQVPMVEI 60
DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA DLEIMILYYP HMPPEEKEAS 120
LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG NRLSRADIAL VELLYHVEEL DPGVVDNFPL 180
LKALRSRVSN LPTVKKFLQP GSQRKPFDDA KCVESAKKIF S 221 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004364; F:glutathione transferase activity; ISS:UniProtKB.
 GO:0006749; P:glutathione metabolic process; ISS:UniProtKB.
 GO:0001657; P:ureteric bud development; IEP:UniProtKB. 
Interpro
 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR003080; GST_alpha.
 IPR004046; GST_C.
 IPR012336; Thioredoxin-like_fold. 
Pfam
 PF00043; GST_C
 PF02798; GST_N 
SMART
  
PROSITE
 PS50405; GST_CTER
 PS50404; GST_NTER 
PRINTS
 PR01266; GSTRNSFRASEA.