CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011212
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 4 gamma 1 
Protein Synonyms/Alias
 eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220 
Gene Name
 EIF4G1 
Gene Synonyms/Alias
 EIF4F; EIF4G; EIF4GI 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
580TWDSKEDKIHNAENIubiquitination[1]
593NIQPGEQKYEYKSDQubiquitination[1, 2]
597GEQKYEYKSDQWKPLubiquitination[1, 2]
609KPLNLEEKKRYDREFubiquitination[1, 3, 4]
770RVRSILNKLTPQMFQubiquitination[1]
795IDTEERLKGVIDLIFubiquitination[1]
832LKVPTTEKPTVTVNFubiquitination[1, 2]
863DDDEVFEKKQKEMDEubiquitination[2]
907KFIGELFKLKMLTEAubiquitination[1, 3, 4]
944RLLTTIGKDLDFEKAubiquitination[3]
1059WNTVPISKGSRPIDTubiquitination[1]
1071IDTSRLTKITKPGSIubiquitination[3]
1095GGRLSWGKGSSGGSGacetylation[5]
1188ATKRSFSKEVEERSRacetylation[5]
1188ATKRSFSKEVEERSRubiquitination[3]
1404REAGLSWKEFLPEGQubiquitination[2]
1587KSVTAFFKWLREAEEubiquitination[1, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
 Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. 
Sequence Annotation
 DOMAIN 565 792 MIF4G.
 DOMAIN 1241 1363 MI.
 DOMAIN 1433 1599 W2.
 REGION 172 200 PABPC1-binding.
 REGION 607 618 EIF4E-binding.
 REGION 682 1085 eIF3/EIF4A-binding.
 REGION 1450 1599 EIF4A-binding.
 REGION 1585 1599 Necessary but not sufficient for MKNK1-
 MOD_RES 205 205 Phosphothreonine (By similarity).
 MOD_RES 207 207 Phosphothreonine.
 MOD_RES 223 223 Phosphothreonine.
 MOD_RES 647 647 Phosphothreonine.
 MOD_RES 1028 1028 Phosphoserine.
 MOD_RES 1092 1092 Phosphoserine.
 MOD_RES 1095 1095 N6-acetyllysine.
 MOD_RES 1145 1145 Phosphoserine.
 MOD_RES 1147 1147 Phosphoserine.
 MOD_RES 1185 1185 Phosphoserine; by PKC/PRKCA.
 MOD_RES 1187 1187 Phosphoserine.
 MOD_RES 1209 1209 Phosphoserine.
 MOD_RES 1211 1211 Phosphothreonine.
 MOD_RES 1231 1231 Phosphoserine.
 MOD_RES 1596 1596 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative initiation; Alternative splicing; Complete proteome; Disease mutation; Host-virus interaction; Initiation factor; Neurodegeneration; Parkinson disease; Parkinsonism; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1599 AA 
Protein Sequence
MNKAPQSTGP PPAPSPGLPQ PAFPPGQTAP VVFSTPQATQ MNTPSQPRQH FYPSRAQPPS 60
SAASRVQSAA PARPGPAAHV YPAGSQVMMI PSQISYPASQ GAYYIPGQGR STYVVPTQQY 120
PVQPGAPGFY PGASPTEFGT YAGAYYPAQG VQQFPTGVAP TPVLMNQPPQ IAPKRERKTI 180
RIRDPNQGGK DITEEIMSGA RTASTPTPPQ TGGGLEPQAN GETPQVAVIV RPDDRSQGAI 240
IADRPGLPGP EHSPSESQPS SPSPTPSPSP VLEPGSEPNL AVLSIPGDTM TTIQMSVEES 300
TPISRETGEP YRLSPEPTPL AEPILEVEVT LSKPVPESEF SSSPLQAPTP LASHTVEIHE 360
PNGMVPSEDL EPEVESSPEL APPPACPSES PVPIAPTAQP EELLNGAPSP PAVDLSPVSE 420
PEEQAKEVTA SMAPPTIPSA TPATAPSATS PAQEEEMEEE EEEEEGEAGE AGEAESEKGG 480
EELLPPESTP IPANLSQNLE AAAATQVAVS VPKRRRKIKE LNKKEAVGDL LDAFKEANPA 540
VPEVENQPPA GSNPGPESEG SGVPPRPEEA DETWDSKEDK IHNAENIQPG EQKYEYKSDQ 600
WKPLNLEEKK RYDREFLLGF QFIFASMQKP EGLPHISDVV LDKANKTPLR PLDPTRLQGI 660
NCGPDFTPSF ANLGRTTLST RGPPRGGPGG ELPRGPAGLG PRRSQQGPRK EPRKIIATVL 720
MTEDIKLNKA EKAWKPSSKR TAADKDRGEE DADGSKTQDL FRRVRSILNK LTPQMFQQLM 780
KQVTQLAIDT EERLKGVIDL IFEKAISEPN FSVAYANMCR CLMALKVPTT EKPTVTVNFR 840
KLLLNRCQKE FEKDKDDDEV FEKKQKEMDE AATAEERGRL KEELEEARDI ARRRSLGNIK 900
FIGELFKLKM LTEAIMHDCV VKLLKNHDEE SLECLCRLLT TIGKDLDFEK AKPRMDQYFN 960
QMEKIIKEKK TSSRIRFMLQ DVLDLRGSNW VPRRGDQGPK TIDQIHKEAE MEEHREHIKV 1020
QQLMAKGSDK RRGGPPGPPI SRGLPLVDDG GWNTVPISKG SRPIDTSRLT KITKPGSIDS 1080
NNQLFAPGGR LSWGKGSSGG SGAKPSDAAS EAARPATSTL NRFSALQQAV PTESTDNRRV 1140
VQRSSLSRER GEKAGDRGDR LERSERGGDR GDRLDRARTP ATKRSFSKEV EERSRERPSQ 1200
PEGLRKAASL TEDRDRGRDA VKREAALPPV SPLKAALSEE ELEKKSKAII EEYLHLNDMK 1260
EAVQCVQELA SPSLLFIFVR HGVESTLERS AIAREHMGQL LHQLLCAGHL STAQYYQGLY 1320
EILELAEDME IDIPHVWLYL AELVTPILQE GGVPMGELFR EITKPLRPLG KAASLLLEIL 1380
GLLCKSMGPK KVGTLWREAG LSWKEFLPEG QDIGAFVAEQ KVEYTLGEES EAPGQRALPS 1440
EELNRQLEKL LKEGSSNQRV FDWIEANLSE QQIVSNTLVR ALMTAVCYSA IIFETPLRVD 1500
VAVLKARAKL LQKYLCDEQK ELQALYALQA LVVTLEQPPN LLRMFFDALY DEDVVKEDAF 1560
YSWESSKDPA EQQGKGVALK SVTAFFKWLR EAEEESDHN 1599 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; TAS:UniProtKB.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0006446; P:regulation of translational initiation; IMP:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR016024; ARM-type_fold.
 IPR003891; Initiation_fac_eIF4g_MI.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3.
 IPR003307; W2_domain. 
Pfam
 PF02847; MA3
 PF02854; MIF4G
 PF02020; W2 
SMART
 SM00515; eIF5C
 SM00544; MA3
 SM00543; MIF4G 
PROSITE
 PS51366; MI
 PS51363; W2 
PRINTS