UniProt Accession

 KAT3_MOUSE; Q71RI9; Q8BJ84 

Genbank Protein ID

 AF363737; AK049569; AK145623; BC131942; BC132615 

Genbank Nucleotide ID

 AAQ15190.1; BAC33817.1; BAE26546.1; AAI31943.1; AAI32616.1 

Protein Name

 Kynurenine--oxoglutarate transaminase 3 

Protein Synonyms/Alias

 Cysteine-S-conjugate beta-lyase 2; Kynurenine aminotransferase III; KATIII; Kynurenine--glyoxylate transaminase; Kynurenine--oxoglutarate transaminase III 

Gene Name

 Ccbl2 

Gene Synonyms/Alias

 Kat3 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
83	ISPPSYVKEELSKAA	ubiquitination	[1]
88	YVKEELSKAAFIDNM	ubiquitination	[1]
109	FGHPALVKALSCLYG	acetylation	[2, 3, 4]
117	ALSCLYGKIYQRQID	acetylation	[2, 3, 4, 5, 6, 7, 8]
117	ALSCLYGKIYQRQID	succinylation	[8]
117	ALSCLYGKIYQRQID	ubiquitination	[1]
182	VFIPLRSKPTDGMKW	acetylation	[2]
204	DPRELESKFSSKTKA	acetylation	[2]
210	SKFSSKTKAIILNTP	ubiquitination	[1]
289	TFSVTGWKLGWSIGP	acetylation	[4]
404	KFVKWMTKHKKLTAI	acetylation	[4]
420	VSAFCDSKSKPHFEK	acetylation	[8]
420	VSAFCDSKSKPHFEK	succinylation	[8]
420	VSAFCDSKSKPHFEK	ubiquitination	[1]
436	VRFCFIKKDSTLDAA	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [8] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). May catalyze the beta-elimination of S-conjugates and Se- conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (By similarity). Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2- oxoglutarate as amino group acceptor (in vitro). 

Sequence Annotation

 BINDING 54 54 Substrate.
 BINDING 72 72 Substrate; via amide nitrogen.
 BINDING 219 219 Substrate.
 BINDING 430 430 Substrate.
 MOD_RES 117 117 N6-acetyllysine (By similarity).
 MOD_RES 281 281 N6-(pyridoxal phosphate)lysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Aminotransferase; Complete proteome; Lyase; Pyridoxal phosphate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 455 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0047804; F:cysteine-S-conjugate beta-lyase activity; IEA:EC.
 GO:0047315; F:kynurenine-glyoxylate transaminase activity; IDA:UniProtKB.
 GO:0016212; F:kynurenine-oxoglutarate transaminase activity; IDA:UniProtKB.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006103; P:2-oxoglutarate metabolic process; IDA:UniProtKB.
 GO:0009058; P:biosynthetic process; IEA:InterPro. 

Interpro

 IPR004839; Aminotransferase_I/II.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 

Pfam

 PF00155; Aminotran_1_2 

SMART

  

PROSITE

  

PRINTS