CPLM-001576

Genbank Nucleotide ID

CUGBP Elav-like family member 2

Protein Synonyms/Alias

CELF-2; Bruno-like protein 3; CUG triplet repeat RNA-binding protein 2; CUG-BP2; CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; ETR-3; Neuroblastoma apoptosis-related RNA-binding protein; hNAPOR; RNA-binding protein BRUNOL-3

BRUNOL3; CUGBP2; ETR3; NAPOR

Homo sapiens (Human)

Position	Peptide	Type	References
458	SAKVFIDKQTNLSKC	acetylation	[1]
458	SAKVFIDKQTNLSKC	ubiquitination	[2]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]

Functional Description

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity).

DOMAIN 40 123 RRM 1.
DOMAIN 132 212 RRM 2.
DOMAIN 423 501 RRM 3.
REGION 1 283 Necessary for RNA-binding, TNNT2 exon 5
REGION 357 508 Necessary for RNA-binding, TNNT2 exon 5

Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; Nucleus; Polymorphism; Reference proteome; Repeat; Repressor; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0003723; F:RNA binding; TAS:ProtInc.
GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
GO:0008016; P:regulation of heart contraction; TAS:ProtInc.
GO:0006396; P:RNA processing; TAS:ProtInc.

IPR002343; Hud_Sxl_RNA.
IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.