CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021941
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UDP-N-acetylglucosamine transferase subunit ALG13 homolog 
Protein Synonyms/Alias
 Asparagine-linked glycosylation 13 homolog; Glycosyltransferase 28 domain-containing protein 1 
Gene Name
 ALG13 
Gene Synonyms/Alias
 CXorf45; GLT28D1; MDS031 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64TLDVYRYKDSLKEDIubiquitination[1]
68YRYKDSLKEDIQKADubiquitination[1]
92SCLETLEKGKPLVVVubiquitination[1]
94LETLEKGKPLVVVINubiquitination[1]
114NHQLELAKQLHKEGHubiquitination[2, 3]
215WKNYCSQKSLNEASMubiquitination[1]
238LFRKLTAKDASCLFRubiquitination[1]
411EDAHTDYKSSNQNRMubiquitination[1]
455SKMPFPYKVLKALDPubiquitination[1]
458PFPYKVLKALDPEIYubiquitination[1]
482DSRKELQKSDYMEYAubiquitination[1]
498RQYYLGDKCQVCLESubiquitination[1]
567RKGRGYQKMPGGYVPubiquitination[1]
683DFYPGPGKRCCQSYDubiquitination[1]
711RQMSCVNKESQYGFTubiquitination[1]
775RRGHSSGKQTLNLEEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Isoform 2 may be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway. 
Sequence Annotation
 DOMAIN 231 352 OTU.
 DOMAIN 492 552 Tudor.  
Keyword
 Alternative splicing; Complete proteome; Congenital disorder of glycosylation; Disease mutation; Endoplasmic reticulum; Glycosyltransferase; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1137 AA 
Protein Sequence
MKCVFVTVGT TSFDDLIACV SAPDSLQKIE SLGYNRLILQ IGRGTVVPEP FSTESFTLDV 60
YRYKDSLKED IQKADLVISH AGAGSCLETL EKGKPLVVVI NEKLMNNHQL ELAKQLHKEG 120
HLFYCTCRVL TCPGQAKSIA SAPGKCQDSA ALTSTAFSGL DFGLLSGYLH KQALVTATHP 180
TCTLLFPSCH AFFPLPLTPT LYKMHKGWKN YCSQKSLNEA SMDEYLGSLG LFRKLTAKDA 240
SCLFRAISEQ LFCSQVHHLE IRKACVSYMR ENQQTFESYV EGSFEKYLER LGDPKESAGQ 300
LEIRALSLIY NRDFILYRFP GKPPTYVTDN GYEDKILLCY SSSGHYDSVY SKQFQSSAAV 360
CQAVLYEILY KDVFVVDEEE LKTAIKLFRS GSKKNRNNAV TGSEDAHTDY KSSNQNRMEE 420
WGACYNAENI PEGYNKGTEE TKSPENPSKM PFPYKVLKAL DPEIYRNVEF DVWLDSRKEL 480
QKSDYMEYAG RQYYLGDKCQ VCLESEGRYY NAHIQEVGNE NNSVTVFIEE LAEKHVVPLA 540
NLKPVTQVMS VPAWNAMPSR KGRGYQKMPG GYVPEIVISE MDIKQQKKMF KKIRGKEVYM 600
TMAYGKGDPL LPPRLQHSMH YGHDPPMHYS QTAGNVMSNE HFHPQHPSPR QGRGYGMPRN 660
SSRFINRHNM PGPKVDFYPG PGKRCCQSYD NFSYRSRSFR RSHRQMSCVN KESQYGFTPG 720
NGQMPRGLEE TITFYEVEEG DETAYPTLPN HGGPSTMVPA TSGYCVGRRG HSSGKQTLNL 780
EEGNGQSENG RYHEEYLYRA EPDYETSGVY STTASTANLS LQDRKSCSMS PQDTVTSYNY 840
PQKMMGNIAA VAASCANNVP APVLSNGAAA NQAISTTSVS SQNAIQPLFV SPPTHGRPVI 900
ASPSYPCHSA IPHAGASLPP PPPPPPPPPP PPPPPPPPPP PPPPPALDVG ETSNLQPPPP 960
LPPPPYSCDP SGSDLPQDTK VLQYYFNLGL QCYYHSYWHS MVYVPQMQQQ LHVENYPVYT 1020
EPPLVDQTVP QCYSEVRRED GIQAEASAND TFPNADSSSV PHGAVYYPVM SDPYGQPPLP 1080
GFDSCLPVVP DYSCVPPWHP VGTAYGGSSQ IHGAINPGPI GCIAPSPPAS HYVPQGM 1137 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0004577; F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity; IEA:EC.
 GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; TAS:Reactome.
 GO:0030259; P:lipid glycosylation; IEA:InterPro.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome. 
Interpro
 IPR007235; Glyco_trans_28_C.
 IPR003323; OTU.
 IPR002999; Tudor. 
Pfam
 PF04101; Glyco_tran_28_C
 PF02338; OTU 
SMART
  
PROSITE
 PS50802; OTU
 PS50304; TUDOR 
PRINTS