CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008122
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 4-trimethylaminobutyraldehyde dehydrogenase 
Protein Synonyms/Alias
 TMABADH; Aldehyde dehydrogenase E3 isozyme; Aldehyde dehydrogenase family 9 member A1; Gamma-aminobutyraldehyde dehydrogenase; R-aminobutyraldehyde dehydrogenase 
Gene Name
 ALDH9A1 
Gene Synonyms/Alias
 ALDH4; ALDH7; ALDH9 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30ADASGTEKAFEPATGacetylation[1]
30ADASGTEKAFEPATGubiquitination[2, 3, 4]
49TFTCSGEKEVNLAVQubiquitination[3]
59NLAVQNAKAAFKIWSubiquitination[4]
68AFKIWSQKSGMERCRubiquitination[3, 4, 5, 6, 7]
239GSVPTGMKIMEMSAKubiquitination[3]
246KIMEMSAKGIKPVTLubiquitination[3]
249EMSAKGIKPVTLELGubiquitination[3]
298GTRVFVQKEILDKFTacetylation[8]
298GTRVFVQKEILDKFTubiquitination[3]
303VQKEILDKFTEEVVKubiquitination[2, 3]
310KFTEEVVKQTQRIKIubiquitination[3, 5]
316VKQTQRIKIGDPLLEubiquitination[3]
344ERVLGFVKVAKEQGAubiquitination[3, 9]
347LGFVKVAKEQGAKVLubiquitination[9]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865] 
Functional Description
 Converts gamma-trimethylaminobutyraldehyde into gamma- butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction. 
Sequence Annotation
 NP_BIND 232 237 NAD (By similarity).
 ACT_SITE 254 254 Proton acceptor (By similarity).
 ACT_SITE 288 288 Nucleophile (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 298 298 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 494 AA 
Protein Sequence
MSTGTFVVSQ PLNYRGGARV EPADASGTEK AFEPATGRVI ATFTCSGEKE VNLAVQNAKA 60
AFKIWSQKSG MERCRILLEA ARIIREREDE IATMECINNG KSIFEARLDI DISWQCLEYY 120
AGLAASMAGE HIQLPGGSFG YTRREPLGVC VGIGAWNYPF QIASWKSAPA LACGNAMVFK 180
PSPFTPVSAL LLAEIYSEAG VPPGLFNVVQ GGAATGQFLC QHPDVAKVSF TGSVPTGMKI 240
MEMSAKGIKP VTLELGGKSP LIIFSDCDMN NAVKGALMAN FLTQGQVCCN GTRVFVQKEI 300
LDKFTEEVVK QTQRIKIGDP LLEDTRMGPL INRPHLERVL GFVKVAKEQG AKVLCGGDIY 360
VPEDPKLKDG YYMRPCVLTN CRDDMTCVKE EIFGPVMSIL SFDTEAEVLE RANDTTFGLA 420
AGVFTRDIQR AHRVVAELQA GTCFINNYNV SPVELPFGGY KKSGFGRENG RVTIEYYSQL 480
KTVCVEMGDV ESAF 494 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0033737; F:1-pyrroline dehydrogenase activity; IEA:EC.
 GO:0004028; F:3-chloroallyl aldehyde dehydrogenase activity; TAS:Reactome.
 GO:0047105; F:4-trimethylammoniobutyraldehyde dehydrogenase activity; IEA:EC.
 GO:0019145; F:aminobutyraldehyde dehydrogenase activity; IDA:UniProtKB.
 GO:0045329; P:carnitine biosynthetic process; TAS:Reactome.
 GO:0006081; P:cellular aldehyde metabolic process; IDA:UniProtKB.
 GO:0042445; P:hormone metabolic process; TAS:UniProtKB.
 GO:0042136; P:neurotransmitter biosynthetic process; IDA:UniProtKB. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016160; Ald_DH_CS.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom. 
Pfam
 PF00171; Aldedh 
SMART
  
PROSITE
 PS00070; ALDEHYDE_DEHYDR_CYS
 PS00687; ALDEHYDE_DEHYDR_GLU 
PRINTS