UniProt Accession

 MDHC_YEAST; P22133; D6W1U2 

Genbank Protein ID

 M62808; U41293; Z74868; BK006948 

Genbank Nucleotide ID

 AAA34766.1; AAC49466.1; CAA99145.1; DAA10658.1 

Protein Name

 Malate dehydrogenase, cytoplasmic 

Protein Synonyms/Alias

  

Gene Name

 MDH2 

Gene Synonyms/Alias

 YOL126C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
42	AQLQYQLKESNRSVT	ubiquitination	[1]
180	RRIMGVTKLDIVRAS	ubiquitination	[1]
254	YGGDEVVKAKNGKGS	ubiquitination	[1]
259	VVKAKNGKGSATLSM	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The isoenzyme MDH2 may function primarily in the glyoxylate cycle. 

Sequence Annotation

 NP_BIND 20 26 NAD (By similarity).
 NP_BIND 144 146 NAD (By similarity).
 ACT_SITE 215 215 Proton acceptor (By similarity).
 BINDING 57 57 NAD (By similarity).
 BINDING 106 106 Substrate (By similarity).
 BINDING 112 112 Substrate (By similarity).
 BINDING 119 119 NAD (By similarity).
 BINDING 146 146 Substrate (By similarity).
 BINDING 185 185 Substrate (By similarity).
 BINDING 266 266 NAD (By similarity).
 MOD_RES 6 6 Phosphothreonine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 377 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0030060; F:L-malate dehydrogenase activity; IDA:SGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006094; P:gluconeogenesis; IMP:SGD.
 GO:0006108; P:malate metabolic process; TAS:SGD.
 GO:0016558; P:protein import into peroxisome matrix; IMP:SGD.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR010097; Malate_DH_type1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00068; MDH 

PRINTS