UniProt Accession

 PCKGC_MOUSE; Q9Z2V4; Q3UEH3 

Genbank Protein ID

 AF009605; AK028046; AK133496; AK149525 

Genbank Nucleotide ID

 AAD01427.1; BAC25718.1; BAE21687.1; BAE28938.1 

Protein Name

 Phosphoenolpyruvate carboxykinase, cytosolic [GTP] 

Protein Synonyms/Alias

 PEPCK-C; Phosphoenolpyruvate carboxylase 

Gene Name

 Pck1 

Gene Synonyms/Alias

 Pepck 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
71	GVIRKLKKYDNCWLA	ubiquitination	[1]
91	DVARIESKTVIITQE	ubiquitination	[1]
107	RDTVPIPKTGLSQLG	ubiquitination	[1]
124	MSEEDFEKAFNARFP	ubiquitination	[1]
135	ARFPGCMKGRTMYVI	ubiquitination	[1]
203	VGCPLPLKKPLVNNW	ubiquitination	[1]
204	GCPLPLKKPLVNNWA	ubiquitination	[1]
243	GGNSLLGKKCFALRI	ubiquitination	[1]
278	TNPEGKKKYLAAAFP	ubiquitination	[1]
290	AFPSACGKTNLAMMN	ubiquitination	[1]
316	GDDIAWMKFDAQGNL	ubiquitination	[1]
342	VAPGTSVKTNPNAIK	ubiquitination	[1]
349	KTNPNAIKTIQKNTI	ubiquitination	[1]
389	TITSWKNKEWRPQDA	ubiquitination	[1]
471	ATAAAEHKGKIIMHD	ubiquitination	[1]
524	FRKDKDGKFLWPGFG	ubiquitination	[1]
551	IEGEDSAKLTPIGYI	ubiquitination	[1]
560	TPIGYIPKENALNLK	ubiquitination	[1]
567	KENALNLKGLGGVNV	ubiquitination	[1]
587	ISKEFWEKEVEEIDR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity). 

Sequence Annotation

 NP_BIND 287 292 GTP (By similarity).
 NP_BIND 530 533 GTP (By similarity).
 REGION 403 405 Substrate binding (By similarity).
 ACT_SITE 288 288 By similarity.
 METAL 244 244 Manganese (By similarity).
 METAL 264 264 Manganese (By similarity).
 METAL 311 311 Manganese (By similarity).
 BINDING 87 87 Substrate (By similarity).
 BINDING 237 237 Substrate; via amide nitrogen (By
 BINDING 244 244 Substrate (By similarity).
 BINDING 286 286 Substrate (By similarity).
 BINDING 405 405 GTP (By similarity).
 BINDING 436 436 GTP (By similarity).
 MOD_RES 70 70 N6-acetyllysine; by p300/EP300 (By
 MOD_RES 71 71 N6-acetyllysine; by p300/EP300 (By  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis; GTP-binding; Lyase; Manganese; Metal-binding; Nucleotide-binding; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 622 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0031406; F:carboxylic acid binding; IEA:Compara.
 GO:0019003; F:GDP binding; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:Compara.
 GO:0030145; F:manganese ion binding; IEA:Compara.
 GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:EC.
 GO:0004611; F:phosphoenolpyruvate carboxykinase activity; IMP:MGI.
 GO:0006094; P:gluconeogenesis; IDA:MGI.
 GO:0042593; P:glucose homeostasis; IEA:Compara.
 GO:0046327; P:glycerol biosynthetic process from pyruvate; IMP:MGI.
 GO:0006475; P:internal protein amino acid acetylation; ISS:UniProtKB.
 GO:0006107; P:oxaloacetate metabolic process; IEA:Compara.
 GO:0014823; P:response to activity; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IEA:Compara. 

Interpro

 IPR018091; PEP_carboxykin_GTP_CS.
 IPR013035; PEP_carboxykinase_C.
 IPR008209; PEP_carboxykinase_GTP.
 IPR008210; PEP_carboxykinase_N. 

Pfam

 PF00821; PEPCK 

SMART

  

PROSITE

 PS00505; PEPCK_GTP 

PRINTS