UniProt Accession

 BRF2_HUMAN; Q9HAW0; B2RD62; D3DSW6; Q9H2Y3; Q9H3B3; Q9NUY6 

Genbank Protein ID

 AF298153; AF206673; AK001914; AK315420; CH471080; CH471080; CH471080; BC010648; AF130058 

Genbank Nucleotide ID

 AAG30222.1; AAG35669.2; BAA91975.1; BAG37809.1; EAW63351.1; EAW63352.1; EAW63353.1; AAH10648.1; AAG35486.1 

Protein Name

 Transcription factor IIIB 50 kDa subunit 

Protein Synonyms/Alias

 TFIIIB50; hTFIIIB50; B-related factor 2; BRF-2; hBRFU 

Gene Name

 BRF2 

Gene Synonyms/Alias

 BRFU; PRO1470 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
192	ASPSVPAKYVEDKEK	ubiquitination	[1, 2]
199	KYVEDKEKMLSRTMQ	ubiquitination	[2]
295	LDKRSVVKHIGDLLQ	ubiquitination	[2, 3]
324	AEVETREKEPPGWGQ	ubiquitination	[1, 2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. 

Sequence Annotation

 REPEAT 72 157 1.
 REPEAT 173 249 2.
 ZN_FING 2 36 TFIIB-type.
 METAL 7 7 Zinc (By similarity).
 METAL 10 10 Zinc (By similarity).
 METAL 28 28 Zinc (By similarity).
 METAL 31 31 Zinc (By similarity).  

Keyword

 Activator; Complete proteome; Metal-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 419 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006383; P:transcription from RNA polymerase III promoter; TAS:Reactome. 

Interpro

 IPR013763; Cyclin-like.
 IPR000812; TFIIB.
 IPR013137; Znf_TFIIB. 

Pfam

 PF08271; TF_Zn_Ribbon 

SMART

 SM00385; CYCLIN 

PROSITE

 PS00782; TFIIB
 PS51134; ZF_TFIIB 

PRINTS