CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004610
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Triosephosphate isomerase 
Protein Synonyms/Alias
 TIM; Triose-phosphate isomerase 
Gene Name
 Tpi1 
Gene Synonyms/Alias
 Tpi 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
56GTMAPTRKFFVGGNWacetylation[1, 2]
56GTMAPTRKFFVGGNWsuccinylation[2]
56GTMAPTRKFFVGGNWubiquitination[3]
64FFVGGNWKMNGRKKCacetylation[1]
64FFVGGNWKMNGRKKCubiquitination[3]
105YIDFARQKLDPKIAVacetylation[2, 4]
109ARQKLDPKIAVAAQNacetylation[1, 2]
109ARQKLDPKIAVAAQNsuccinylation[2]
109ARQKLDPKIAVAAQNubiquitination[3]
119VAAQNCYKVTNGAFTubiquitination[3]
135EISPGMIKDLGATWVacetylation[1]
192REAGITEKVVFEQTKacetylation[4]
192REAGITEKVVFEQTKubiquitination[3]
199KVVFEQTKVIADNVKubiquitination[3]
206KVIADNVKDWSKVVLacetylation[4]
206KVIADNVKDWSKVVLubiquitination[3]
210DNVKDWSKVVLAYEPubiquitination[3]
225VWAIGTGKTATPQQAubiquitination[3]
238QAQEVHEKLRGWLKSacetylation[4]
238QAQEVHEKLRGWLKSubiquitination[3]
244EKLRGWLKSNVNDGVubiquitination[3]
288LVGGASLKPEFVDIIubiquitination[3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
  
Sequence Annotation
 ACT_SITE 146 146 Electrophile (By similarity).
 ACT_SITE 216 216 Proton acceptor (By similarity).
 BINDING 62 62 Substrate (By similarity).
 BINDING 64 64 Substrate (By similarity).
 MOD_RES 64 64 N6-acetyllysine (By similarity).
 MOD_RES 118 118 Nitrated tyrosine.
 MOD_RES 130 130 Phosphoserine (By similarity).
 MOD_RES 244 244 N6-acetyllysine (By similarity).
 MOD_RES 259 259 Nitrated tyrosine.
 MOD_RES 288 288 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Gluconeogenesis; Glycolysis; Isomerase; Nitration; Pentose shunt; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 299 AA 
Protein Sequence
MEGKAEQQGA GLTMAEGGEK EEFCFTAIYI SGQWREPCVC TDLQRLEPGT MAPTRKFFVG 60
GNWKMNGRKK CLGELICTLN AANVPAGTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV 120
TNGAFTGEIS PGMIKDLGAT WVVLGHSERR HVFGESDELI GQKVSHALAE GLGVIACIGE 180
KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR 240
GWLKSNVNDG VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ 299 
Gene Ontology
 GO:0004807; F:triose-phosphate isomerase activity; IDA:MGI.
 GO:0009790; P:embryo development; IMP:MGI.
 GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
 GO:0006006; P:glucose metabolic process; IDA:MGI.
 GO:0019682; P:glyceraldehyde-3-phosphate metabolic process; IDA:UniProtKB.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
 GO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-KW. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR022896; TrioseP_Isoase_bac/euk.
 IPR000652; Triosephosphate_isomerase.
 IPR020861; Triosephosphate_isomerase_AS. 
Pfam
 PF00121; TIM 
SMART
  
PROSITE
 PS00171; TIM_1
 PS51440; TIM_2 
PRINTS