CPLM-018564

Genbank Nucleotide ID

Protein Synonyms/Alias

Hypoxia-inducible factor prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; Prolyl hydroxylase domain-containing protein 2; PHD2; SM-20

Mus musculus (Mouse)

Position	Peptide	Type	References
385	VKYLTGEKGVRVELK	acetylation	[1]

[1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]

Functional Description

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality.

DOMAIN 271 369 Fe2OG dioxygenase.
ZN_FING 21 58 MYND-type.
REGION 6 20 Required for nuclear export.
REGION 218 228 Beta(2)beta(3) 'finger-like' loop (By
METAL 290 290 Iron (By similarity).
METAL 292 292 Iron (By similarity).
METAL 351 351 Iron (By similarity).
BINDING 360 360 2-oxoglutarate (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 178 178 S-nitrosocysteine (By similarity).
MOD_RES 185 185 S-nitrosocysteine (By similarity).
MOD_RES 279 279 S-nitrosocysteine (By similarity).
MOD_RES 300 300 S-nitrosocysteine (By similarity).
MOD_RES 303 303 S-nitrosocysteine (By similarity).

Acetylation; Complete proteome; Cytoplasm; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Reference proteome; S-nitrosylation; Vitamin C; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IEA:Compara.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005506; F:iron ion binding; IEA:InterPro.
GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
GO:0031545; F:peptidyl-proline 4-dioxygenase activity; IEA:Compara.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0055008; P:cardiac muscle tissue morphogenesis; IMP:MGI.
GO:0060347; P:heart trabecula formation; IMP:MGI.
GO:0060711; P:labyrinthine layer development; IMP:MGI.
GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IEA:Compara.
GO:0032364; P:oxygen homeostasis; IEA:Compara.
GO:0018401; P:peptidyl-proline hydroxylation to 4-hydroxy-L-proline; IEA:Compara.
GO:0045765; P:regulation of angiogenesis; IMP:UniProtKB.
GO:0001666; P:response to hypoxia; IEA:Compara.
GO:0071731; P:response to nitric oxide; ISS:UniProtKB.
GO:0060412; P:ventricular septum morphogenesis; IMP:MGI.

IPR005123; Oxoglu/Fe-dep_dioxygenase.
IPR006620; Pro_4_hyd_alph.
IPR002893; Znf_MYND.

PF13640; 2OG-FeII_Oxy_3
PF01753; zf-MYND

PS51471; FE2OG_OXY
PS01360; ZF_MYND_1
PS50865; ZF_MYND_2