CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006408
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peroxisomal targeting signal receptor 
Protein Synonyms/Alias
 PTS1 receptor; PTS1R; Peroxin-5; Peroxisomal protein PAS10 
Gene Name
 PEX5 
Gene Synonyms/Alias
 PAS10; YDR244W; YD8419.11 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
18NPLAQLHKHTQQNKSubiquitination[1, 2]
24HKHTQQNKSLQFNQKubiquitination[1, 2]
46SPLQGTNKPGISEAFubiquitination[3]
Reference
 [1] Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling.
 Platta HW, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R.
 J Cell Biol. 2007 Apr 23;177(2):197-204. [PMID: 17452527]
 [2] A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p.
 Williams C, van den Berg M, Sprenger RR, Distel B.
 J Biol Chem. 2007 Aug 3;282(31):22534-43. [PMID: 17550898]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. 
Sequence Annotation
 REPEAT 64 97 TPR 1.
 REPEAT 313 346 TPR 2.
 REPEAT 347 380 TPR 3.
 REPEAT 381 418 TPR 4.
 REPEAT 419 456 TPR 5.
 REPEAT 457 490 TPR 6.
 REPEAT 491 524 TPR 7.
 REPEAT 525 558 TPR 8.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 61 61 Phosphoserine.
 CROSSLNK 6 6 Glycyl cysteine thioester (Cys-Gly)
 CROSSLNK 18 18 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 24 24 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Isopeptide bond; Membrane; Peroxisome; Phosphoprotein; Protein transport; Reference proteome; Repeat; Thioester bond; TPR repeat; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 612 AA 
Protein Sequence
MDVGSCSVGN NPLAQLHKHT QQNKSLQFNQ KNNGRLNESP LQGTNKPGIS EAFISNVNAI 60
SQENMANMQR FINGEPLIDD KRRMEIGPSS GRLPPFSNVH SLQTSANPTQ IKGVNDISHW 120
SQEFQGSNSI QNRNADTGNS EKAWQRGSTT ASSRFQYPNT MMNNYAYASM NSLSGSRLQS 180
PAFMNQQQSG RSKEGVNEQE QQPWTDQFEK LEKEVSENLD INDEIEKEEN VSEVEQNKPE 240
TVEKEEGVYG DQYQSDFQEV WDSIHKDAEE VLPSELVNDD LNLGEDYLKY LGGRVNGNIE 300
YAFQSNNEYF NNPNAYKIGC LLMENGAKLS EAALAFEAAV KEKPDHVDAW LRLGLVQTQN 360
EKELNGISAL EECLKLDPKN LEAMKTLAIS YINEGYDMSA FTMLDKWAET KYPEIWSRIK 420
QQDDKFQKEK GFTHIDMNAH ITKQFLQLAN NLSTIDPEIQ LCLGLLFYTK DDFDKTIDCF 480
ESALRVNPND ELMWNRLGAS LANSNRSEEA IQAYHRALQL KPSFVRARYN LAVSSMNIGC 540
FKEAAGYLLS VLSMHEVNTN NKKGDVGSLL NTYNDTVIET LKRVFIAMNR DDLLQEVKPG 600
MDLKRFKGEF SF 612 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005778; C:peroxisomal membrane; IDA:SGD.
 GO:0005052; F:peroxisome matrix targeting signal-1 binding; IDA:SGD.
 GO:0016560; P:protein import into peroxisome matrix, docking; IMP:SGD. 
Interpro
 IPR024113; PTS1R.
 IPR024111; PTS1R_family.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF13181; TPR_8 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS