CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016788
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 von Willebrand factor A domain-containing protein 8 
Protein Synonyms/Alias
  
Gene Name
 Vwa8 
Gene Synonyms/Alias
 Kiaa0564 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
220KLLQDHTKEELDAWKacetylation[1]
359MAVEGVLKRFELQGSacetylation[2]
555RYLSLKEKLQLTDEQubiquitination[3]
988NIVKHLQKFPTEGLSacetylation[1]
988NIVKHLQKFPTEGLSubiquitination[3]
1074ETNHVDIKGPVLVNMacetylation[1]
1083PVLVNMEKYPIEKHEacetylation[4]
1083PVLVNMEKYPIEKHEubiquitination[3]
1351LTSAVGQKIASPNRIacetylation[1]
1584DTAGLGGKGGPYRLDubiquitination[3]
1628KAFQQRLKEIQMSEYubiquitination[3]
1757ENYEEKFKYDIAGHSacetylation[4]
Reference
 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123
Functional Description
  
Sequence Annotation
 DOMAIN 1714 1896 VWFA.
 NP_BIND 446 453 ATP (Potential).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome; Secreted; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1905 AA 
Protein Sequence
MQSRLLLLGA PGGLGDVASR RVRLLLRQVL RGRPGGDQQR LEVRLLHSGA TDSGETVSIG 60
DVSYKLKTPK NPELVPQNYI SDSPAQSIVQ HLRWLMQKDL LGQDVFLIGP PGPLRRSVAM 120
QYLELTKREV EYIALSRDTT ETDLKQRREI RAGTAFYIDQ CAVRAATEGR TLVLEGLEKA 180
ERNVLPVLNN LLENREMQLE DGRFLMSAER YDKLLQDHTK EELDAWKIVR VSENFRVIAL 240
GLPVPRYSGN PLDPPLRSRF QARDIYFLPF QDQLKLLYSV GANVSAEKIS QLLSFATTLC 300
SQESSTLGLP DFPLDSLPEA VQILDSFPMM SIEHALQWVY PYTLLLGHEG KMAVEGVLKR 360
FELQGSGHSL LPKEIVRVER MTDSHGSYAH VTIRVAGKEV TIKVPAGTRA VNQPCAPDHF 420
IQTVSHKQLL AEMVQSHMVK DICLIGGKGC GKTVIAKNFA ALLGYSIEPI MLYQDMTARD 480
LLQQRYTLPN GDTAWRSSPL VSAAREGKLV LLDGIHRVNA GTLAVLQRLI HDRELSLYDG 540
SRLLREDRYL SLKEKLQLTD EQLQNRSIFP IHPSFRIIAL AEPPIVGSTT QQWLGPEFLT 600
MFFFHHMKPL VKSEEIQVIK ETVPNVPQEA LEKLLSVTHK LRETQDPTAQ SLAASLSTRQ 660
LLRISRRLSK YPSENLHDAI TKACLSRFLP SLAQSALEKN LADAAIETNT EDSLEPELEN 720
YKCEVVAGSL KIGAVSVPVH NAHEKMKVPD VLFYDNVQHM VVMEDMLKDF VLGEHLLLVG 780
NQGVGKNKIV DRFLHLLNRP REYIQLHRDT TVQSLTLQPT VKGGLIVYED SPLVKAVKLG 840
HILVVDEADK APTNVTCILK TLVENGEMIL ADGRRIVADA ANVDGRENLV AIHPDFRMLA 900
LANRPGFPFL GNDFFGTLGD IFSCHAIDNP KPHSELSMLK QYGPDVPEPV LQKLVAAFGE 960
LRNLADQGII NYPYSTREVV NIVKHLQKFP TEGLSSVVRN VFDFDSYNND MREILMNTLH 1020
KYGIPIGAKP TNVQLAKEFP LPEKTFMGYW IVGQTGNGMQ KVLCPAETNH VDIKGPVLVN 1080
MEKYPIEKHE ARFLSFTEEC TSWKFPLDEV NLICDIAVSH ENGEQTLYVA ACNPVSLYFM 1140
NMTGKNGFFV DFFDIFPRMA SGSWRPFVTV APLGSPLRGQ VVLHEEQSNA VLLLDTTGSA 1200
IRRLVLPTEE FTSKKSSWWS KEEGETYRMC KEFSHKNWVV FYKQTGNSLT VLDVLEGLAH 1260
TISLPINLRT VFLVAEDKWL LVENETNQKY LLTKPAHIGS EDTGACQLYM LKEELPSTGF 1320
GVTQETEFCI PDKVSSDQLS SENLTSAVGQ KIASPNRILS DENSHATIVV GFPDLMSPSE 1380
VYSWKRSSSL RQPSVTSMTM YTGKRTNATP RHNNCVTLTH TNQVVRILPP GEVPLKDLYP 1440
KDVTPPQTAG YIEVTDLQAK KLRYIPVPSA ESLSPYTAWI SAISDTDALL AEWDKSSVVT 1500
VDMGGRVRLW ETGLERLQQS LMEWRNMIGQ DSDKHVQITI ERDSNEDVSD PKHGKEDPDN 1560
MPHVGGNTWA GGTGGRDTAG LGGKGGPYRL DAGHPVYQVS EVEKDAVPED VKRAAREMAQ 1620
KAFQQRLKEI QMSEYDAATY ERFSSAVQRQ VHALRIILDN LQAKGKERQW LRHQATGELD 1680
DAKIIDGLAG EKSIYKRRGD LEPQLGSPQQ KPKRLRLVVD VSGSMYRFNG VDRRLERSME 1740
AVCMVMEAFE NYEEKFKYDI AGHSGDGYNI KLVPVNQIPK NNKQRLEILK TMHEHSQFCM 1800
SGDHTLEGTE HAIKDITTEE ADEYFVIILS DANLSRYGIN PARFAQILTS DPQVNAFAIF 1860
IGSLGDQAAR LQRTLPAGRS FIAMDTKKIP QILQQIFTST MLSSI 1905 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IEA:InterPro.
 GO:0006200; P:ATP catabolic process; IEA:GOC. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR011704; ATPase_dyneun-rel_AAA.
 IPR027417; P-loop_NTPase.
 IPR002035; VWF_A. 
Pfam
 PF07728; AAA_5 
SMART
 SM00382; AAA
 SM00327; VWA 
PROSITE
 PS00674; AAA
 PS50234; VWFA 
PRINTS