CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000298
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neuropilin-1 
Protein Synonyms/Alias
 Vascular endothelial cell growth factor 165 receptor; CD304 
Gene Name
 NRP1 
Gene Synonyms/Alias
 NRP; VEGF165R 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53HSYHPSEKCEWLIQAubiquitination[1]
106FRGKFCGKIAPPPVVubiquitination[1]
744RVKLRYQKPEEYDQLubiquitination[2]
764GHQGDHWKEGRVLLHubiquitination[2]
913GVKLKKDKLNTQSTYubiquitination[2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. 
Sequence Annotation
 DOMAIN 27 141 CUB 1.
 DOMAIN 147 265 CUB 2.
 DOMAIN 275 424 F5/8 type C 1.
 DOMAIN 431 583 F5/8 type C 2.
 DOMAIN 645 811 MAM.
 METAL 195 195 Calcium.
 METAL 209 209 Calcium.
 METAL 250 250 Calcium.
 CARBOHYD 150 150 N-linked (GlcNAc...).
 CARBOHYD 261 261 N-linked (GlcNAc...).
 CARBOHYD 300 300 N-linked (GlcNAc...) (Potential).
 CARBOHYD 522 522 N-linked (GlcNAc...).
 CARBOHYD 612 612 O-linked (Xyl...) (chondroitin sulfate);
 CARBOHYD 612 612 O-linked (Xyl...) (heparan sulfate);
 CARBOHYD 842 842 N-linked (GlcNAc...) (Potential).
 DISULFID 27 54 Probable.
 DISULFID 82 104 Probable.
 DISULFID 147 173
 DISULFID 206 228
 DISULFID 275 424
 DISULFID 431 583  
Keyword
 3D-structure; Alternative splicing; Angiogenesis; Calcium; Cell membrane; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Disulfide bond; Glycoprotein; Heparan sulfate; Heparin-binding; Membrane; Metal-binding; Neurogenesis; Polymorphism; Proteoglycan; Receptor; Reference proteome; Repeat; Secreted; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 923 AA 
Protein Sequence
MERGLPLLCA VLALVLAPAG AFRNDKCGDT IKIESPGYLT SPGYPHSYHP SEKCEWLIQA 60
PDPYQRIMIN FNPHFDLEDR DCKYDYVEVF DGENENGHFR GKFCGKIAPP PVVSSGPFLF 120
IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTTPSGVIKS PGFPEKYPNS LECTYIVFVP 180
KMSEIILEFE SFDLEPDSNP PGGMFCRYDR LEIWDGFPDV GPHIGRYCGQ KTPGRIRSSS 240
GILSMVFYTD SAIAKEGFSA NYSVLQSSVS EDFKCMEALG MESGEIHSDQ ITASSQYSTN 300
WSAERSRLNY PENGWTPGED SYREWIQVDL GLLRFVTAVG TQGAISKETK KKYYVKTYKI 360
DVSSNGEDWI TIKEGNKPVL FQGNTNPTDV VVAVFPKPLI TRFVRIKPAT WETGISMRFE 420
VYGCKITDYP CSGMLGMVSG LISDSQITSS NQGDRNWMPE NIRLVTSRSG WALPPAPHSY 480
INEWLQIDLG EEKIVRGIII QGGKHRENKV FMRKFKIGYS NNGSDWKMIM DDSKRKAKSF 540
EGNNNYDTPE LRTFPALSTR FIRIYPERAT HGGLGLRMEL LGCEVEAPTA GPTTPNGNLV 600
DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTVIDSTIQ SEFPTYGFNC EFGWGSHKTF 660
CHWEHDNHVQ LKWSVLTSKT GPIQDHTGDG NFIYSQADEN QKGKVARLVS PVVYSQNSAH 720
CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ LVWMAIGHQG DHWKEGRVLL HKSLKLYQVI 780
FEGEIGKGNL GGIAVDDISI NNHISQEDCA KPADLDKKNP EIKIDETGST PGYEGEGEGD 840
KNISRKPGNV LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN 900
FELVDGVKLK KDKLNTQSTY SEA 923 
Gene Ontology
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0031410; C:cytoplasmic vesicle; TAS:BHF-UCL.
 GO:0005829; C:cytosol; IDA:BHF-UCL.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005883; C:neurofilament; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:BHF-UCL.
 GO:0002116; C:semaphorin receptor complex; NAS:BHF-UCL.
 GO:0015026; F:coreceptor activity; TAS:BHF-UCL.
 GO:0019955; F:cytokine binding; NAS:BHF-UCL.
 GO:0019838; F:growth factor binding; TAS:BHF-UCL.
 GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0017154; F:semaphorin receptor activity; NAS:BHF-UCL.
 GO:0005021; F:vascular endothelial growth factor-activated receptor activity; NAS:BHF-UCL.
 GO:0007411; P:axon guidance; NAS:BHF-UCL.
 GO:0007413; P:axonal fasciculation; IEA:Compara.
 GO:0007155; P:cell adhesion; IEA:InterPro.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0035729; P:cellular response to hepatocyte growth factor stimulus; IMP:BHF-UCL.
 GO:0016358; P:dendrite development; IEA:Compara.
 GO:0060666; P:dichotomous subdivision of terminal units involved in salivary gland branching; IEA:Compara.
 GO:0035767; P:endothelial cell chemotaxis; IMP:BHF-UCL.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0048012; P:hepatocyte growth factor receptor signaling pathway; IMP:BHF-UCL.
 GO:0048843; P:negative regulation of axon extension involved in axon guidance; IEA:Compara.
 GO:0009887; P:organ morphogenesis; TAS:ProtInc.
 GO:0001569; P:patterning of blood vessels; IEA:Compara.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; IMP:BHF-UCL.
 GO:0060301; P:positive regulation of cytokine activity; TAS:BHF-UCL.
 GO:0010595; P:positive regulation of endothelial cell migration; TAS:BHF-UCL.
 GO:0001938; P:positive regulation of endothelial cell proliferation; TAS:BHF-UCL.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IMP:BHF-UCL.
 GO:0014911; P:positive regulation of smooth muscle cell migration; TAS:BHF-UCL.
 GO:0060627; P:regulation of vesicle-mediated transport; TAS:BHF-UCL.
 GO:0009611; P:response to wounding; IEA:Compara.
 GO:0048485; P:sympathetic nervous system development; IEA:Compara.
 GO:0021636; P:trigeminal nerve morphogenesis; IEA:Compara.
 GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; IMP:BHF-UCL. 
Interpro
 IPR000421; Coagulation_fac_5/8-C_type_dom.
 IPR008985; ConA-like_lec_gl_sf.
 IPR000859; CUB_dom.
 IPR008979; Galactose-bd-like.
 IPR000998; MAM_dom.
 IPR014648; Neuropilin.
 IPR022579; Neuropilin1_C.
 IPR027146; NRP1. 
Pfam
 PF00431; CUB
 PF11980; DUF3481
 PF00754; F5_F8_type_C
 PF00629; MAM 
SMART
 SM00042; CUB
 SM00231; FA58C
 SM00137; MAM 
PROSITE
 PS01180; CUB
 PS01285; FA58C_1
 PS01286; FA58C_2
 PS50022; FA58C_3
 PS00740; MAM_1
 PS50060; MAM_2 
PRINTS
 PR00020; MAMDOMAIN.