UniProt Accession

 TMX3_HUMAN; Q96JJ7; B3KV75; Q52LT7; Q8N5J0; Q9NWJ9 

Genbank Protein ID

 AB058733; AK000800; AK122715; BX647846; BC032325; BC093792; BC093794 

Genbank Nucleotide ID

 BAB47459.1; BAA91381.1; BAG53687.1; AAH32325.1; AAH93792.1; AAH93794.1 

Protein Name

 Protein disulfide-isomerase TMX3 

Protein Synonyms/Alias

 Thioredoxin domain-containing protein 10; Thioredoxin-related transmembrane protein 3 

Gene Name

 TMX3 

Gene Synonyms/Alias

 KIAA1830; TXNDC10 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
58	PWCGHCKKLEPIWNE	ubiquitination	[1]
71	NEVGLEMKSIGSPVK	ubiquitination	[1]
78	KSIGSPVKVGKMDAT	ubiquitination	[1, 2]
81	GSPVKVGKMDATSYS	ubiquitination	[1]
106	YPTIKLLKGDLAYNY	ubiquitination	[1]
119	NYRGPRTKDDIIEFA	ubiquitination	[1]
166	GESPLKEKYIDAASE	ubiquitination	[1]
267	SVEHTRLKSIIQEVA	ubiquitination	[1, 3, 4]
364	KRIVFDAKSTIVSIF	ubiquitination	[1, 2]
414	EERYEVSKSENENQE	ubiquitination	[2, 3, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Probable disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May act as a dithiol oxidase. 

Sequence Annotation

 DOMAIN 25 128 Thioredoxin.
 MOTIF 451 454 Di-lysine motif.
 CARBOHYD 258 258 N-linked (GlcNAc...).
 CARBOHYD 313 313 N-linked (GlcNAc...) (Potential).
 DISULFID 53 56 Redox-active (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Isomerase; Membrane; Polymorphism; Redox-active center; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 454 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:MGI.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0003756; F:protein disulfide isomerase activity; IEA:EC.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0006662; P:glycerol ether metabolic process; IEA:InterPro.
 GO:0006457; P:protein folding; IEA:GOC. 

Interpro

 IPR005746; Thioredoxin.
 IPR012336; Thioredoxin-like_fold.
 IPR017937; Thioredoxin_CS.
 IPR013766; Thioredoxin_domain. 

Pfam

 PF00085; Thioredoxin 

SMART

  

PROSITE

 PS00194; THIOREDOXIN_1
 PS51352; THIOREDOXIN_2 

PRINTS

 PR00421; THIOREDOXIN.