UniProt Accession

 AZI2_HUMAN; Q9H6S1; A8K3M2; C9JB40; H7BXU6; Q86W99; Q9BQF1 

Genbank Protein ID

 AY151386; AF044917; AF044918; AK025592; AK290637; AC092503; AC098616; CH471055 

Genbank Nucleotide ID

 AAO05967.1; AAF22368.1; AAF22369.1; BAB15182.1; BAF83326.1; EAW64395.1 

Protein Name

 5-azacytidine-induced protein 2 

Protein Synonyms/Alias

 NF-kappa-B-activating kinase-associated protein 1; Nak-associated protein 1; TILP 

Gene Name

 AZI2 

Gene Synonyms/Alias

 NAP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
194	KIELQKAKQTDPYQE	ubiquitination	[1]
205	PYQEDNLKSRDLQKL	ubiquitination	[1, 2]
255	KTSTAIKKACAPVGC	ubiquitination	[1, 3]
293	PPLLPNGKALCHTTS	ubiquitination	[1]
313	DVKVLSEKAILQSWT	ubiquitination	[1, 2]
366	ETAFGETKTKTLPLP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1. 

Sequence Annotation

 REGION 158 270 Interaction with TBK1.
 MOD_RES 353 353 Phosphoserine.  

Keyword

 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 392 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; IEA:Compara. 

Interpro

 IPR024581; TBD. 

Pfam

 PF12845; TBD 

SMART

  

PROSITE

  

PRINTS