CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002378
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin alpha-V 
Protein Synonyms/Alias
 Vitronectin receptor subunit alpha; CD51; Integrin alpha-V heavy chain; Integrin alpha-V light chain 
Gene Name
 ITGAV 
Gene Synonyms/Alias
 MSK8; VNRA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
233DPNVYSIKYNNQLATubiquitination[1, 2, 3, 4, 5, 6]
360SGDFQTTKLNGFEVFubiquitination[1, 2, 3, 4, 5, 6, 7]
399APYGGEDKKGIVYIFubiquitination[2, 5]
446KGATDIDKNGYPDLIubiquitination[2, 5]
581DESEFRDKLTPITIFubiquitination[2, 4, 5]
645VSVDSDQKKIYIGDDubiquitination[2, 5]
646SVDSDQKKIYIGDDNubiquitination[4, 5]
718CDLGNPMKAGTQLLAubiquitination[5]
755QSSNLFDKVSPVVSHubiquitination[5]
873SSLQTTEKNDTVAGQubiquitination[2]
889ERDHLITKRDLALSEubiquitination[2]
923VGRLDRGKSAILYVKubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. 
Sequence Annotation
 REPEAT 32 98 FG-GAP 1.
 REPEAT 109 170 FG-GAP 2.
 REPEAT 173 225 FG-GAP 3.
 REPEAT 237 295 FG-GAP 4.
 REPEAT 296 357 FG-GAP 5.
 REPEAT 358 415 FG-GAP 6.
 REPEAT 419 482 FG-GAP 7.
 MOTIF 1019 1023 GFFKR motif.
 CARBOHYD 74 74 N-linked (GlcNAc...).
 CARBOHYD 290 290 N-linked (GlcNAc...) (Potential).
 CARBOHYD 296 296 N-linked (GlcNAc...) (Potential).
 CARBOHYD 488 488 N-linked (GlcNAc...) (Potential).
 CARBOHYD 554 554 N-linked (GlcNAc...) (Potential).
 CARBOHYD 615 615 N-linked (GlcNAc...).
 CARBOHYD 704 704 N-linked (GlcNAc...).
 CARBOHYD 835 835 N-linked (GlcNAc...) (Potential).
 CARBOHYD 851 851 N-linked (GlcNAc...) (Potential).
 CARBOHYD 874 874 N-linked (GlcNAc...).
 CARBOHYD 945 945 N-linked (GlcNAc...) (Potential).
 CARBOHYD 973 973 N-linked (GlcNAc...) (Potential).
 CARBOHYD 980 980 N-linked (GlcNAc...) (Potential).
 DISULFID 89 97
 DISULFID 138 158
 DISULFID 172 185
 DISULFID 491 502
 DISULFID 508 565
 DISULFID 626 632
 DISULFID 698 711
 DISULFID 852 914 Interchain (between heavy and light
 DISULFID 904 909  
Keyword
 3D-structure; Alternative splicing; Calcium; Cell adhesion; Cleavage on pair of basic residues; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Host cell receptor for virus entry; Host-virus interaction; Integrin; Membrane; Metal-binding; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1048 AA 
Protein Sequence
MAFPPRRRLR LGPRGLPLLL SGLLLPLCRA FNLDVDSPAE YSGPEGSYFG FAVDFFVPSA 60
SSRMFLLVGA PKANTTQPGI VEGGQVLKCD WSSTRRCQPI EFDATGNRDY AKDDPLEFKS 120
HQWFGASVRS KQDKILACAP LYHWRTEMKQ EREPVGTCFL QDGTKTVEYA PCRSQDIDAD 180
GQGFCQGGFS IDFTKADRVL LGGPGSFYWQ GQLISDQVAE IVSKYDPNVY SIKYNNQLAT 240
RTAQAIFDDS YLGYSVAVGD FNGDGIDDFV SGVPRAARTL GMVYIYDGKN MSSLYNFTGE 300
QMAAYFGFSV AATDINGDDY ADVFIGAPLF MDRGSDGKLQ EVGQVSVSLQ RASGDFQTTK 360
LNGFEVFARF GSAIAPLGDL DQDGFNDIAI AAPYGGEDKK GIVYIFNGRS TGLNAVPSQI 420
LEGQWAARSM PPSFGYSMKG ATDIDKNGYP DLIVGAFGVD RAILYRARPV ITVNAGLEVY 480
PSILNQDNKT CSLPGTALKV SCFNVRFCLK ADGKGVLPRK LNFQVELLLD KLKQKGAIRR 540
ALFLYSRSPS HSKNMTISRG GLMQCEELIA YLRDESEFRD KLTPITIFME YRLDYRTAAD 600
TTGLQPILNQ FTPANISRQA HILLDCGEDN VCKPKLEVSV DSDQKKIYIG DDNPLTLIVK 660
AQNQGEGAYE AELIVSIPLQ ADFIGVVRNN EALARLSCAF KTENQTRQVV CDLGNPMKAG 720
TQLLAGLRFS VHQQSEMDTS VKFDLQIQSS NLFDKVSPVV SHKVDLAVLA AVEIRGVSSP 780
DHVFLPIPNW EHKENPETEE DVGPVVQHIY ELRNNGPSSF SKAMLHLQWP YKYNNNTLLY 840
ILHYDIDGPM NCTSDMEINP LRIKISSLQT TEKNDTVAGQ GERDHLITKR DLALSEGDIH 900
TLGCGVAQCL KIVCQVGRLD RGKSAILYVK SLLWTETFMN KENQNHSYSL KSSASFNVIE 960
FPYKNLPIED ITNSTLVTTN VTWGIQPAPM PVPVWVIILA VLAGLLLLAV LVFVMYRMGF 1020
FKRVRPPQEE QEREQLQPHE NGEGNSET 1048 
Gene Ontology
 GO:0035867; C:alphav-beta3 integrin-IGF-1-IGF1R complex; IDA:BHF-UCL.
 GO:0009986; C:cell surface; IDA:BHF-UCL.
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0008305; C:integrin complex; NAS:UniProtKB.
 GO:0045335; C:phagocytic vesicle; TAS:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050431; F:transforming growth factor beta binding; ISS:BHF-UCL.
 GO:0005245; F:voltage-gated calcium channel activity; IDA:UniProtKB.
 GO:0001525; P:angiogenesis; IEP:UniProtKB.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0043277; P:apoptotic cell clearance; IEA:Compara.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007160; P:cell-matrix adhesion; NAS:UniProtKB.
 GO:0052066; P:entry of symbiont into host cell by promotion of host phagocytosis; NAS:BHF-UCL.
 GO:0070371; P:ERK1 and ERK2 cascade; ISS:BHF-UCL.
 GO:0030198; P:extracellular matrix organization; TAS:Reactome.
 GO:0007229; P:integrin-mediated signaling pathway; NAS:UniProtKB.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0043066; P:negative regulation of apoptotic process; IDA:BHF-UCL.
 GO:2000536; P:negative regulation of entry of bacterium into host cell; IDA:BHF-UCL.
 GO:0010888; P:negative regulation of lipid storage; IMP:BHF-UCL.
 GO:0032369; P:negative regulation of lipid transport; IMP:BHF-UCL.
 GO:0050748; P:negative regulation of lipoprotein metabolic process; IMP:BHF-UCL.
 GO:0045715; P:negative regulation of low-density lipoprotein particle receptor biosynthetic process; IMP:BHF-UCL.
 GO:0010745; P:negative regulation of macrophage derived foam cell differentiation; IMP:BHF-UCL.
 GO:0045785; P:positive regulation of cell adhesion; IDA:BHF-UCL.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IDA:BHF-UCL.
 GO:0033690; P:positive regulation of osteoblast proliferation; IEA:Compara.
 GO:2000425; P:regulation of apoptotic cell clearance; ISS:BHF-UCL.
 GO:0046718; P:viral entry into host cell; TAS:BHF-UCL. 
Interpro
 IPR013517; FG-GAP.
 IPR013519; Int_alpha_beta-p.
 IPR000413; Integrin_alpha.
 IPR013649; Integrin_alpha-2.
 IPR018184; Integrin_alpha_C_CS. 
Pfam
 PF01839; FG-GAP
 PF00357; Integrin_alpha
 PF08441; Integrin_alpha2 
SMART
 SM00191; Int_alpha 
PROSITE
 PS51470; FG_GAP
 PS00242; INTEGRIN_ALPHA 
PRINTS
 PR01185; INTEGRINA.