CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000700
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tumor protein D54 
Protein Synonyms/Alias
 hD54; Tumor protein D52-like 2 
Gene Name
 TPD52L2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55ELRAELTKVEEEIVTubiquitination[1]
90LSTLGELKQNLSRSWubiquitination[2, 3, 4, 5, 6, 7]
108QVSSAYVKTSEKLGEubiquitination[2, 3, 4, 5, 7, 8, 9]
112AYVKTSEKLGEWNEKubiquitination[3, 6, 8, 9]
119KLGEWNEKVTQSDLYubiquitination[3]
128TQSDLYKKTQETLSQubiquitination[3, 6, 9, 10]
139TLSQAGQKTSAALSTubiquitination[3, 11]
165MRNSATFKSFEDRVGubiquitination[5]
Reference
 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [11] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266
Functional Description
  
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 12 12 Phosphoserine.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 21 21 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 149 149 Phosphoserine.
 MOD_RES 166 166 Phosphoserine.
 MOD_RES 192 192 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 206 AA 
Protein Sequence
MDSAGQDINL NSPNKGLLSD SMTDVPVDTG VAARTPAVEG LTEAEEEELR AELTKVEEEI 60
VTLRQVLAAK ERHCGELKRR LGLSTLGELK QNLSRSWHDV QVSSAYVKTS EKLGEWNEKV 120
TQSDLYKKTQ ETLSQAGQKT SAALSTVGSA ISRKLGDMRN SATFKSFEDR VGTIKSKVVG 180
DRENGSDNLP SSAGSGDKPL SDPAPF 206 
Gene Ontology
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; TAS:UniProtKB. 
Interpro
 IPR007327; TPD52. 
Pfam
 PF04201; TPD52 
SMART
  
PROSITE
  
PRINTS