CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000393
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phospholipid scramblase 1 
Protein Synonyms/Alias
 PL scramblase 1; Ca(2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b 
Gene Name
 PLSCR1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
132TNNKYEIKNSFGQRVubiquitination[1]
223KFTIQNEKREDVLKIubiquitination[1, 2, 3]
261CVVGKISKHWTGILRubiquitination[2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. 
Sequence Annotation
 MOTIF 18 26 SH3-binding 1 (Potential).
 MOTIF 22 25 WW-binding 1 (Potential).
 MOTIF 33 36 WW-binding 2 (Potential).
 MOTIF 42 50 SH3-binding 2 (Potential).
 MOTIF 84 92 SH3-binding 3 (Potential).
 MOTIF 257 266 Nuclear localization signal.
 MOD_RES 69 69 Phosphotyrosine; by ABL.
 MOD_RES 74 74 Phosphotyrosine; by ABL.
 MOD_RES 161 161 Phosphothreonine; by PKC.
 LIPID 184 184 S-palmitoyl cysteine (Probable).
 LIPID 185 185 S-palmitoyl cysteine (Probable).
 LIPID 186 186 S-palmitoyl cysteine (Potential).
 LIPID 188 188 S-palmitoyl cysteine (Probable).
 LIPID 189 189 S-palmitoyl cysteine (Probable).  
Keyword
 3D-structure; Antiviral defense; Calcium; Complete proteome; Direct protein sequencing; DNA-binding; Lipoprotein; Membrane; Nucleus; Palmitate; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH3-binding; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 318 AA 
Protein Sequence
MDKQNSQMNA SHPETNLPVG YPPQYPPTAF QGPPGYSGYP GPQVSYPPPP AGHSGPGPAG 60
FPVPNQPVYN QPVYNQPVGA AGVPWMPAPQ PPLNCPPGLE YLSQIDQILI HQQIELLEVL 120
TGFETNNKYE IKNSFGQRVY FAAEDTDCCT RNCCGPSRPF TLRIIDNMGQ EVITLERPLR 180
CSSCCCPCCL QEIEIQAPPG VPIGYVIQTW HPCLPKFTIQ NEKREDVLKI SGPCVVCSCC 240
GDVDFEIKSL DEQCVVGKIS KHWTGILREA FTDADNFGIQ FPLDLDVKMK AVMIGACFLI 300
DFMFFESTGS QEQKSGVW 318 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031012; C:extracellular matrix; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; IDA:UniProtKB.
 GO:0045121; C:membrane raft; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0017128; F:phospholipid scramblase activity; IDA:UniProtKB.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:UniProtKB.
 GO:0017124; F:SH3 domain binding; IDA:UniProtKB.
 GO:0006953; P:acute-phase response; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IDA:UniProtKB.
 GO:0051607; P:defense response to virus; IMP:UniProtKB.
 GO:0045071; P:negative regulation of viral genome replication; IMP:UniProtKB.
 GO:0006659; P:phosphatidylserine biosynthetic process; ISS:UniProtKB.
 GO:0017121; P:phospholipid scrambling; IDA:UniProtKB.
 GO:0030168; P:platelet activation; NAS:UniProtKB.
 GO:2000373; P:positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity; IDA:UniProtKB.
 GO:0045089; P:positive regulation of innate immune response; IMP:UniProtKB.
 GO:0060368; P:regulation of Fc receptor mediated stimulatory signaling pathway; ISS:UniProtKB.
 GO:0033003; P:regulation of mast cell activation; ISS:UniProtKB.
 GO:0035456; P:response to interferon-beta; IMP:UniProtKB. 
Interpro
 IPR005552; Scramblase. 
Pfam
 PF03803; Scramblase 
SMART
  
PROSITE
  
PRINTS