CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022057
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sialin 
Protein Synonyms/Alias
 H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular H(+)/Aspartate-glutamate cotransporter 
Gene Name
 SLC17A5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
263KRISHYEKEYILSSLubiquitination[1, 2]
278RNQLSSQKSVPWVPIubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential- dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO(3)(-)/H(+) cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva. 
Sequence Annotation
 MOTIF 22 23 Dileucine internalization motif.
 CARBOHYD 71 71 N-linked (GlcNAc...) (Potential).
 CARBOHYD 77 77 N-linked (GlcNAc...) (Potential).
 CARBOHYD 95 95 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Amino-acid transport; Cell junction; Cell membrane; Complete proteome; Cytoplasmic vesicle; Disease mutation; Glycoprotein; Lysosome; Membrane; Reference proteome; Symport; Synapse; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 495 AA 
Protein Sequence
MRSPVRDLAR NDGEESTDRT PLLPGAPRAE AAPVCCSARY NLAILAFFGF FIVYALRVNL 60
SVALVDMVDS NTTLEDNRTS KACPEHSAPI KVHHNQTGKK YQWDAETQGW ILGSFFYGYI 120
ITQIPGGYVA SKIGGKMLLG FGILGTAVLT LFTPIAADLG VGPLIVLRAL EGLGEGVTFP 180
AMHAMWSSWA PPLERSKLLS ISYAGAQLGT VISLPLSGII CYYMNWTYVF YFFGTIGIFW 240
FLLWIWLVSD TPQKHKRISH YEKEYILSSL RNQLSSQKSV PWVPILKSLP LWAIVVAHFS 300
YNWTFYTLLT LLPTYMKEIL RFNVQENGFL SSLPYLGSWL CMILSGQAAD NLRAKWNFST 360
LCVRRIFSLI GMIGPAVFLV AAGFIGCDYS LAVAFLTIST TLGGFCSSGF SINHLDIAPS 420
YAGILLGITN TFATIPGMVG PVIAKSLTPD NTVGEWQTVF YIAAAINVFG AIFFTLFAKG 480
EVQNWALNDH HGHRH 495 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005765; C:lysosomal membrane; IDA:MGI.
 GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0015136; F:sialic acid transmembrane transporter activity; IDA:MGI.
 GO:0005351; F:sugar:hydrogen symporter activity; TAS:ProtInc.
 GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
 GO:0015739; P:sialic acid transport; IDA:MGI. 
Interpro
 IPR011701; MFS.
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt. 
Pfam
 PF07690; MFS_1 
SMART
  
PROSITE
 PS50850; MFS 
PRINTS