UniProt Accession

 NEDD8_RAT; Q71UE8 

Genbank Protein ID

 AF095740; BC084728 

Genbank Nucleotide ID

 AAC64189.1; AAH84728.1 

Protein Name

 NEDD8 

Protein Synonyms/Alias

 Neddylin; Ubiquitin-like protein NEDD8 

Gene Name

 Nedd8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
4	****MLIKVKTLTGK	acetylation	[1]
11	KVKTLTGKEIEIDIE	acetylation	[1]
11	KVKTLTGKEIEIDIE	ubiquitination	[2]
22	IDIEPTDKVERIKER	acetylation	[1]
22	IDIEPTDKVERIKER	ubiquitination	[2]
27	TDKVERIKERVEEKE	ubiquitination	[2]
48	QRLIYSGKQMNDEKT	acetylation	[1]
48	QRLIYSGKQMNDEKT	ubiquitination	[2]
54	GKQMNDEKTAADYKI	acetylation	[1]
54	GKQMNDEKTAADYKI	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (By similarity). 

Sequence Annotation

 REGION 70 72 Interaction with UBE1C (By similarity).
 CROSSLNK 76 76 Glycyl lysine isopeptide (Gly-Lys)  

Keyword

 Complete proteome; Direct protein sequencing; Isopeptide bond; Nucleus; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 81 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:RGD.
 GO:0008104; P:protein localization; IEA:Compara.
 GO:0045116; P:protein neddylation; IDA:RGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IDA:RGD. 

Interpro

 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019956; Ubiquitin_subgr.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF00240; ubiquitin 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 

PRINTS

 PR00348; UBIQUITIN.