CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030738
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2 subunit 1 
Protein Synonyms/Alias
 cDNA FLJ57962, highly similar to Homo sapiens eukaryotic translation initiation factor (eIF) 2A (eIF2A), mRNA 
Gene Name
 EIF2A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67NIISVTNKGLLHSFDubiquitination[1, 2]
108ANKLHLQKINDFVLSubiquitination[1, 2, 3]
122SPGPQPYKVAVYVPGubiquitination[1, 2, 3]
131AVYVPGSKGAPSFVRubiquitination[1, 2, 3, 4, 5, 6]
155PHAALANKSFFKADKubiquitination[2, 3]
244KATIFNLKCDPVFDFubiquitination[1]
286QMEVWDVKNYKLISKubiquitination[3]
289VWDVKNYKLISKPVAubiquitination[1]
293KNYKLISKPVASDSTubiquitination[1]
380EVPNEEPKVATAYRPubiquitination[1, 3]
474SGDPEIDKKIKNLKKacetylation[7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Initiation factor; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 524 AA 
Protein Sequence
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRESGKNCK VCIFSKDGTL FAWGNGEKVN 60
IISVTNKGLL HSFDLLKAVC LEFSPKNTVL ATWQPYTNTI ANKLHLQKIN DFVLSPGPQP 120
YKVAVYVPGS KGAPSFVRLY QYPNFAGPHA ALANKSFFKA DKVTMLWNKK ATAVLVIAST 180
DVDKTGASYY GEQTLHYIAT NGESAVVQLP KNGPIYDVVW NSSSTEFCAV YGFMPAKATI 240
FNLKCDPVFD FGTGPRNAAY YSPHGHILVL AGFGNLRGQM EVWDVKNYKL ISKPVASDST 300
YFAWCPDGEH ILTATCAPRL RVNNGYKIWH YTGSILHKYD VPSNAELWQV SWQPFLDGIF 360
PAKTITYQAV PSEVPNEEPK VATAYRPPAL RNKPITNSKL HEEEPPQNMK PQSGNDKPLS 420
KTALKNQRKH EAKKAAKQEA RSDKSPDLAP TPAPQSTPRN TVSQSISGDP EIDKKIKNLK 480
KKLKAIEQLK EQAATGKQLE KNQLEKIQKE TALLQELEDL ELGI 524 
Gene Ontology
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. 
Interpro
 IPR011387; TIF2A.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF08662; eIF2A 
SMART
  
PROSITE
  
PRINTS