CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005774
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AT-rich interactive domain-containing protein 4A 
Protein Synonyms/Alias
 ARID domain-containing protein 4A; Retinoblastoma-binding protein 1; RBBP-1 
Gene Name
 ARID4A 
Gene Synonyms/Alias
 RBBP1; RBP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
109RRTSLCLKGERHFAEubiquitination[1]
345YKDLNLFKLFRLVYHubiquitination[1]
418HHHEPKVKEEKKDLEsumoylation[2]
444EMPLTEVKSEPEENIsumoylation[2]
867LGQSSPEKKIRIENGacetylation[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] RBP1 family proteins exhibit SUMOylation-dependent transcriptional repression and induce cell growth inhibition reminiscent of senescence.
 Binda O, Roy JS, Branton PE.
 Mol Cell Biol. 2006 Mar;26(5):1917-31. [PMID: 16479010]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Interacts with the viral protein-binding domain of the retinoblastoma protein. 
Sequence Annotation
 DOMAIN 309 401 ARID.
 REGION 951 964 Retinoblastoma protein binding
 MOD_RES 538 538 Phosphoserine.
 MOD_RES 542 542 Phosphoserine.
 MOD_RES 676 676 Phosphoserine.
 MOD_RES 1109 1109 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1257 AA 
Protein Sequence
MKAADEPAYL TVGTDVSAKY RGAFCEAKIK TVKRLVKVKV LLKQDNTTQL VQDDQVKGPL 60
RVGAIVETRT SDGSFQEAII SKLTDASWYT VVFDDGDERT LRRTSLCLKG ERHFAESETL 120
DQLPLTNPEH FGTPVIAKKT NRGRRSSLPV TEDEKEEESS EEEDEDKRRL NDELLGKVVS 180
VVSATERTEW YPALVISPSC NDDITVKKDQ CLVRSFIDSK FYSIARKDIK EVDILNLPES 240
ELSTKPGLQK ASIFLKTRVV PDNWKMDISE ILESSSSDDE DGPAEENDEE KEKEAKKTEE 300
EVPEEELDPE ERDNFLQQLY KFMEDRGTPI NKPPVLGYKD LNLFKLFRLV YHQGGCDNID 360
SGAVWKQIYM DLGIPILNSA ASYNVKTAYR KYLYGFEEYC RSANIQFRTV HHHEPKVKEE 420
KKDLEESMEE ALKLDQEMPL TEVKSEPEEN IDSNSESERE EIELKSPRGR RRIARDVNSI 480
KKEIEEEKTE DKLKDNDTEN KDVDDDYETA EKKENELLLG RKNTPKQKEK KIKKQEDSDK 540
DSDEEEEKSQ EREETESKCD SEGEEDEEDM EPCLTGTKVK VKYGRGKTQK IYEASIKSTE 600
IDDGEVLYLV HYYGWNVRYD EWVKADRIIW PLDKGGPKKK QKKKAKNKED SEKDEKRDEE 660
RQKSKRGRPP LKSTLSSNMP YGLSKTANSE GKSDSCSSDS ETEDALEKNL INEELSLKDE 720
LEKNENLNDD KLDEENPKIS AHILKENDRT QMQPLETLKL EVGENEQIVQ IFGNKMEKTE 780
EVKKEAEKSP KGKGRRSKTK DLSLEIIKIS SFGQNEAGSE PHIEAHSLEL SSLDNKNFSS 840
ATEDEIDQCV KEKKLKRKIL GQSSPEKKIR IENGMEMTNT VSQERTSDCI GSEGMKNLNF 900
EQHFERENEG MPSLIAESNQ CIQQLTSERF DSPAEETVNI PLKEDEDAMP LIGPETLVCH 960
EVDLDDLDEK DKTSIEDVAV ESSESNSLVS IPPALPPVVQ HNFSVASPLT LSQDESRSVK 1020
SESDITIEVD SIAEESQEGL CERESANGFE TNVASGTCSI IVQERESREK GQKRPSDGNS 1080
GLMAKKQKRT PKRTSAAAKN EKNGTGQSSD SEDLPVLDNS SKCTPVKHLN VSKPQKLARS 1140
PARISPHIKD GEKDKHREKH PNSSPRTYKW SFQLNELDNM NSTERISFLQ EKLQEIRKYY 1200
MSLKSEVATI DRRRKRLKKK DREVSHAGAS MSSASSDTGM SPSSSSPPQN VLAVECR 1257 
Gene Ontology
 GO:0017053; C:transcriptional repressor complex; IDA:GDB.
 GO:0003677; F:DNA binding; IDA:GDB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:GDB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR001606; ARID/BRIGHT_DNA-bd.
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR012603; RBB1NT.
 IPR002999; Tudor.
 IPR025995; Tudor-knot. 
Pfam
 PF01388; ARID
 PF08169; RBB1NT
 PF11717; Tudor-knot 
SMART
 SM00501; BRIGHT
 SM00298; CHROMO
 SM00333; TUDOR 
PROSITE
 PS51011; ARID 
PRINTS