CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012560
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Scaffold attachment factor B1 
Protein Synonyms/Alias
 SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein 
Gene Name
 SAFB 
Gene Synonyms/Alias
 HAP; HET; SAFB1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83EITSEGNKKTSKRSSubiquitination[1]
231TCKSEPVKEESSELEsumoylation[2]
231TCKSEPVKEESSELEubiquitination[1]
294DSLLAVVKREPAEQPsumoylation[2]
356DSKEDGRKFDFDACNubiquitination[3]
381TSEGADQKMSSPEDDubiquitination[1]
423TTRATDLKNLFSKYGubiquitination[3, 4]
428DLKNLFSKYGKVVGAubiquitination[4]
436YGKVVGAKVVTNARSubiquitination[3, 4]
475HKTELHGKMISVEKAacetylation[5]
607RSVVSFDKVKEPRKSacetylation[5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Co-repressor activity of scaffold attachment factor B1 requires sumoylation.
 Garee JP, Meyer R, Oesterreich S.
 Biochem Biophys Res Commun. 2011 May 20;408(4):516-22. [PMID: 21527249]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330] 
Functional Description
 Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation. 
Sequence Annotation
 DOMAIN 31 65 SAP.
 DOMAIN 406 484 RRM.
 REGION 528 792 Interaction with POLR2A.
 REGION 599 915 Interaction with SAFB2.
 MOTIF 599 616 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 55 55 Phosphoserine.
 MOD_RES 383 383 Phosphoserine.
 MOD_RES 384 384 Phosphoserine.
 MOD_RES 415 415 Phosphoserine.
 MOD_RES 580 580 Phosphoserine.
 MOD_RES 582 582 Phosphoserine.
 MOD_RES 601 601 Phosphoserine.
 MOD_RES 604 604 Phosphoserine.
 MOD_RES 607 607 N6-acetyllysine.
 CROSSLNK 231 231 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 294 294 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-binding; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 915 AA 
Protein Sequence
MAETLSGLGD SGAAGAAALS SASSETGTRR LSDLRVIDLR AELRKRNVDS SGNKSVLMER 60
LKKAIEDEGG NPDEIEITSE GNKKTSKRSS KGRKPEEEGV EDNGLEENSG DGQEDVETSL 120
ENLQDIDIMD ISVLDEAEID NGSVADCVED DDADNLQESL SDSRELVEGE MKELPEQLQE 180
HAIEDKETIN NLDTSSSDFT ILQEIEEPSL EPENEKILDI LGETCKSEPV KEESSELEQP 240
FAQDTSSVGP DRKLAEEEDL FDSAHPEEGD LDLASESTAH AQSSKADSLL AVVKREPAEQ 300
PGDGERTDCE PVGLEPAVEQ SSAASELAEA SSEELAEAPT EAPSPEARDS KEDGRKFDFD 360
ACNEVPPAPK ESSTSEGADQ KMSSPEDDSD TKRLSKEEKG RSSCGRNFWV SGLSSTTRAT 420
DLKNLFSKYG KVVGAKVVTN ARSPGARCYG FVTMSTAEEA TKCINHLHKT ELHGKMISVE 480
KAKNEPVGKK TSDKRDSDGK KEKSSNSDRS TNLKRDDKCD RKDDAKKGDD GSGEKSKDQD 540
DQKPGPSERS RATKSGSRGT ERTVVMDKSK GVPVISVKTS GSKERASKSQ DRKSASREKR 600
SVVSFDKVKE PRKSRDSESH SRVRERSERE QRMQAQWERE ERERLEIARE RLAFQRQRLE 660
RERMERERLE RERMHVEHER RREQERIHRE REELRRQQEL RYEQERRPAV RRPYDLDRRD 720
DAYWPEAKRA ALDERYHSDF NRQDRFHDFD HRDRGRYPDH SVDRREGSRS MMGEREGQHY 780
PERHGGPERH GRDSRDGWGG YGSDKRMSEG RGLPPPPRRD WGDHGRREDD RSWQGTADGG 840
MMDRDHKRWQ GGERSMSGHS GPGHMMNRGG MSGRGSFAPG GASRGHPIPH GGMQGGFGGQ 900
SRGSRPSDAR FTRRY 915 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0001047; F:core promoter binding; IEA:Compara.
 GO:0003690; F:double-stranded DNA binding; TAS:ProtInc.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006325; P:chromatin organization; TAS:ProtInc.
 GO:0040007; P:growth; IEA:Compara.
 GO:0042445; P:hormone metabolic process; IEA:Compara.
 GO:0030520; P:intracellular estrogen receptor signaling pathway; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR003034; SAP_dom. 
Pfam
 PF02037; SAP 
SMART
 SM00360; RRM
 SM00513; SAP 
PROSITE
 PS50102; RRM
 PS50800; SAP 
PRINTS