UniProt Accession

 PRKRA_HUMAN; O75569; A8K3I6; Q53G24; Q6X7T5; Q8NDK4 

Genbank Protein ID

 AF072860; AF083033; AY251164; AL136615; AL833867; BT007243; AK290601; CR533525; AK223107; AC009948; CH471058; BC009470 

Genbank Nucleotide ID

 AAC25672.1; AAD33099.1; AAP20061.1; CAB66550.1; CAD38725.1; AAP35907.1; BAF83290.1; CAG38556.1; BAD96827.1; AAX88882.1; EAX11036.1; AAH09470.1 

Protein Name

 Interferon-inducible double stranded RNA-dependent protein kinase activator A 

Protein Synonyms/Alias

 PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double stranded RNA-dependent activator 

Gene Name

 PRKRA 

Gene Synonyms/Alias

 PACT; RAX; HSD-14; HSD14 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
84	CTGEGTSKKLAKHRA	ubiquitination	[1]
85	TGEGTSKKLAKHRAA	ubiquitination	[2]
173	ESFMETGKGASKKQA	ubiquitination	[2]
177	ETGKGASKKQAKRNA	ubiquitination	[2]
187	AKRNAAEKFLAKFSN	ubiquitination	[1, 2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity). 

Sequence Annotation

 DOMAIN 34 101 DRBM 1.
 DOMAIN 126 194 DRBM 2.
 DOMAIN 240 308 DRBM 3.
 REGION 1 103 Sufficient for self-association and
 REGION 102 195 Sufficient for self-association and
 REGION 195 313 Sufficient for self-association and
 MOD_RES 18 18 Phosphoserine.
 MOD_RES 246 246 Phosphoserine.
 MOD_RES 287 287 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Dystonia; Parkinsonism; Phosphoprotein; Reference proteome; Repeat; RNA-binding; RNA-mediated gene silencing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 313 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0008047; F:enzyme activator activity; TAS:ProtInc.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0042474; P:middle ear morphogenesis; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0042473; P:outer ear morphogenesis; IEA:Compara.
 GO:0030422; P:production of siRNA involved in RNA interference; IDA:UniProtKB.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0006950; P:response to stress; IEA:Compara.
 GO:0009615; P:response to virus; TAS:ProtInc.
 GO:0048705; P:skeletal system morphogenesis; IEA:Compara. 

Interpro

 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom. 

Pfam

 PF00035; dsrm 

SMART

 SM00358; DSRM 

PROSITE

 PS50137; DS_RBD 

PRINTS