CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002691
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin alpha-5 
Protein Synonyms/Alias
 CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain 
Gene Name
 ITGA5 
Gene Synonyms/Alias
 FNRA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
137GEEPVEYKSLQWFGAubiquitination[1, 2]
166LYSWRTEKEPLSDPVubiquitination[3]
943LHFRVWAKTFLQREHubiquitination[3]
1038PYGTAMEKAQLKPPAubiquitination[4]
1042AMEKAQLKPPATSDAubiquitination[1, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. 
Sequence Annotation
 REPEAT 43 108 FG-GAP 1.
 REPEAT 128 188 FG-GAP 2.
 REPEAT 192 245 FG-GAP 3.
 REPEAT 259 315 FG-GAP 4.
 REPEAT 316 377 FG-GAP 5.
 REPEAT 378 437 FG-GAP 6.
 REPEAT 441 504 FG-GAP 7.
 REGION 1021 1028 Interaction with HPS5.
 MOTIF 1024 1028 GFFKR motif.
 METAL 280 280 Calcium 1.
 METAL 282 282 Calcium 1.
 METAL 284 284 Calcium 1.
 METAL 286 286 Calcium 1; via carbonyl oxygen.
 METAL 288 288 Calcium 1.
 METAL 334 334 Calcium 2.
 METAL 336 336 Calcium 2.
 METAL 338 338 Calcium 2.
 METAL 340 340 Calcium 2; via carbonyl oxygen.
 METAL 342 342 Calcium 2.
 METAL 401 401 Calcium 3.
 METAL 403 403 Calcium 3.
 METAL 405 405 Calcium 3.
 METAL 407 407 Calcium 3; via carbonyl oxygen.
 METAL 409 409 Calcium 3.
 METAL 465 465 Calcium 4.
 METAL 467 467 Calcium 4.
 METAL 469 469 Calcium 4.
 METAL 471 471 Calcium 4; via carbonyl oxygen.
 METAL 473 473 Calcium 4.
 BINDING 262 262 Arg of R-G-D substrate.
 BINDING 269 269 Arg of R-G-D substrate.
 CARBOHYD 84 84 N-linked (GlcNAc...).
 CARBOHYD 182 182 N-linked (GlcNAc...).
 CARBOHYD 297 297 N-linked (GlcNAc...).
 CARBOHYD 307 307 N-linked (GlcNAc...).
 CARBOHYD 316 316 N-linked (GlcNAc...).
 CARBOHYD 524 524 N-linked (GlcNAc...) (Potential).
 CARBOHYD 530 530 N-linked (GlcNAc...) (Potential).
 CARBOHYD 593 593 N-linked (GlcNAc...) (Potential).
 CARBOHYD 609 609 N-linked (GlcNAc...).
 CARBOHYD 675 675 N-linked (GlcNAc...).
 CARBOHYD 712 712 N-linked (GlcNAc...) (Potential).
 CARBOHYD 724 724 N-linked (GlcNAc...) (Potential).
 CARBOHYD 771 771 N-linked (GlcNAc...); atypical.
 CARBOHYD 773 773 N-linked (GlcNAc...).
 CARBOHYD 868 868 N-linked (GlcNAc...) (Potential).
 DISULFID 99 108
 DISULFID 156 176
 DISULFID 192 205
 DISULFID 513 522
 DISULFID 528 584
 DISULFID 645 651
 DISULFID 718 731
 DISULFID 869 921 Interchain (between heavy and light
 DISULFID 911 916  
Keyword
 3D-structure; Calcium; Cell adhesion; Cleavage on pair of basic residues; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Host-virus interaction; Integrin; Membrane; Metal-binding; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1049 AA 
Protein Sequence
MGSRTPESPL HAVQLRWGPR RRPPLLPLLL LLLPPPPRVG GFNLDAEAPA VLSGPPGSFF 60
GFSVEFYRPG TDGVSVLVGA PKANTSQPGV LQGGAVYLCP WGASPTQCTP IEFDSKGSRL 120
LESSLSSSEG EEPVEYKSLQ WFGATVRAHG SSILACAPLY SWRTEKEPLS DPVGTCYLST 180
DNFTRILEYA PCRSDFSWAA GQGYCQGGFS AEFTKTGRVV LGGPGSYFWQ GQILSATQEQ 240
IAESYYPEYL INLVQGQLQT RQASSIYDDS YLGYSVAVGE FSGDDTEDFV AGVPKGNLTY 300
GYVTILNGSD IRSLYNFSGE QMASYFGYAV AATDVNGDGL DDLLVGAPLL MDRTPDGRPQ 360
EVGRVYVYLQ HPAGIEPTPT LTLTGHDEFG RFGSSLTPLG DLDQDGYNDV AIGAPFGGET 420
QQGVVFVFPG GPGGLGSKPS QVLQPLWAAS HTPDFFGSAL RGGRDLDGNG YPDLIVGSFG 480
VDKAVVYRGR PIVSASASLT IFPAMFNPEE RSCSLEGNPV ACINLSFCLN ASGKHVADSI 540
GFTVELQLDW QKQKGGVRRA LFLASRQATL TQTLLIQNGA REDCREMKIY LRNESEFRDK 600
LSPIHIALNF SLDPQAPVDS HGLRPALHYQ SKSRIEDKAQ ILLDCGEDNI CVPDLQLEVF 660
GEQNHVYLGD KNALNLTFHA QNVGEGGAYE AELRVTAPPE AEYSGLVRHP GNFSSLSCDY 720
FAVNQSRLLV CDLGNPMKAG ASLWGGLRFT VPHLRDTKKT IQFDFQILSK NLNNSQSDVV 780
SFRLSVEAQA QVTLNGVSKP EAVLFPVSDW HPRDQPQKEE DLGPAVHHVY ELINQGPSSI 840
SQGVLELSCP QALEGQQLLY VTRVTGLNCT TNHPINPKGL ELDPEGSLHH QQKREAPSRS 900
SASSGPQILK CPEAECFRLR CELGPLHQQE SQSLQLHFRV WAKTFLQREH QPFSLQCEAV 960
YKALKMPYRI LPRQLPQKER QVATAVQWTK AEGSYGVPLW IIILAILFGL LLLGLLIYIL 1020
YKLGFFKRSL PYGTAMEKAQ LKPPATSDA 1049 
Gene Ontology
 GO:0071062; C:alphav-beta3 integrin-vitronectin complex; TAS:BHF-UCL.
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0008305; C:integrin complex; TAS:ProtInc.
 GO:0001726; C:ruffle; TAS:HGNC.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005161; F:platelet-derived growth factor receptor binding; TAS:BHF-UCL.
 GO:0043184; F:vascular endothelial growth factor receptor 2 binding; TAS:BHF-UCL.
 GO:0001525; P:angiogenesis; TAS:BHF-UCL.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0033631; P:cell-cell adhesion mediated by integrin; IEA:Compara.
 GO:0007044; P:cell-substrate junction assembly; IEA:Compara.
 GO:0030198; P:extracellular matrix organization; TAS:Reactome.
 GO:0007157; P:heterophilic cell-cell adhesion; IEA:Compara.
 GO:0007229; P:integrin-mediated signaling pathway; IEA:UniProtKB-KW.
 GO:0007159; P:leukocyte cell-cell adhesion; IEA:Compara.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0007613; P:memory; IEA:Compara.
 GO:2000811; P:negative regulation of anoikis; IMP:UniProtKB.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IMP:BHF-UCL.
 GO:0030949; P:positive regulation of vascular endothelial growth factor receptor signaling pathway; TAS:BHF-UCL.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.
 GO:0035313; P:wound healing, spreading of epidermal cells; IEP:BHF-UCL. 
Interpro
 IPR013517; FG-GAP.
 IPR013519; Int_alpha_beta-p.
 IPR000413; Integrin_alpha.
 IPR013649; Integrin_alpha-2.
 IPR018184; Integrin_alpha_C_CS. 
Pfam
 PF01839; FG-GAP
 PF08441; Integrin_alpha2 
SMART
 SM00191; Int_alpha 
PROSITE
 PS51470; FG_GAP
 PS00242; INTEGRIN_ALPHA 
PRINTS
 PR01185; INTEGRINA.