| Tag | Content |
|---|
| CPLM ID | CPLM-013448 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Kelch-like protein 12 |
Protein Synonyms/Alias | CUL3-interacting protein 1; DKIR homolog; hDKIR |
Gene Name | KLHL12 |
Gene Synonyms/Alias | C3IP1 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 157 | LLQLKGVKQACCEFL | ubiquitination | [1] | | 294 | RDLVDEAKKFHLRPE | ubiquitination | [1] | | 295 | DLVDEAKKFHLRPEL | ubiquitination | [1] | | 343 | VVEKYDPKTQEWSFL | ubiquitination | [1] | | 500 | NVTPMATKRSGAGVA | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. |
Sequence Annotation | DOMAIN 33 100 BTB.
DOMAIN 135 236 BACK.
REPEAT 282 329 Kelch 1.
REPEAT 331 379 Kelch 2.
REPEAT 380 426 Kelch 3.
REPEAT 427 473 Kelch 4.
REPEAT 475 520 Kelch 5.
REPEAT 522 567 Kelch 6.
REGION 405 568 Interaction with DVL3. |
Keyword | 3D-structure; Alternative splicing; Complete proteome; Cytoplasmic vesicle; ER-Golgi transport; Kelch repeat; Polymorphism; Reference proteome; Repeat; Transport; Ubl conjugation pathway; Wnt signaling pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 568 AA |
Protein Sequence | MDVNKFEASV GFLDVKKFLS TWKLQNPRTH FVLSPHCFMG GIMAPKDIMT NTHAKSILNS 60
MNSLRKSNTL CDVTLRVEQK DFPAHRIVLA ACSDYFCAMF TSELSEKGKP YVDIQGLTAS 120
TMEILLDFVY TETVHVTVEN VQELLPAACL LQLKGVKQAC CEFLESQLDP SNCLGIRDFA 180
ETHNCVDLMQ AAEVFSQKHF PEVVQHEEFI LLSQGEVEKL IKCDEIQVDS EEPVFEAVIN 240
WVKHAKKERE ESLPNLLQYV RMPLLTPRYI TDVIDAEPFI RCSLQCRDLV DEAKKFHLRP 300
ELRSQMQGPR TRARLGANEV LLVVGGFGSQ QSPIDVVEKY DPKTQEWSFL PSITRKRRYV 360
ASVSLHDRIY VIGGYDGRSR LSSVECLDYT ADEDGVWYSV APMNVRRGLA GATTLGDMIY 420
VSGGFDGSRR HTSMERYDPN IDQWSMLGDM QTAREGAGLV VASGVIYCLG GYDGLNILNS 480
VEKYDPHTGH WTNVTPMATK RSGAGVALLN DHIYVVGGFD GTAHLSSVEA YNIRTDSWTT 540
VTSMTTPRCY VGATVLRGRL YAIAGYDGNS LLSSIECYDP IIDSWEVVTS MGTQRCDAGV 600
CVLREK 606 |
Gene Ontology | GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB. GO:0030134; C:ER to Golgi transport vesicle; IDA:UniProtKB. GO:0048208; P:COPII vesicle coating; IMP:UniProtKB. GO:0006513; P:protein monoubiquitination; IDA:UniProtKB. GO:0016055; P:Wnt receptor signaling pathway; IMP:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |