CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003186
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lon protease 
Protein Synonyms/Alias
 ATP-dependent protease La 
Gene Name
 lon 
Gene Synonyms/Alias
 capR; deg; lopA; muc; b0439; JW0429 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
89KLPDGTVKVLVEGLQacetylation[1, 2]
113NGEHFSAKAEYLESPacetylation[2]
143SQFEGYIKLNKKIPPacetylation[2]
176IAAHMPLKLADKQSVacetylation[2]
235YYLNEQMKAIQKELGacetylation[2]
239EQMKAIQKELGEMDDacetylation[2]
255PDENEALKRKIDAAKacetylation[2]
405YIGSMPGKLIQKMAKacetylation[2]
409MPGKLIQKMAKVGVKacetylation[1, 2]
412KLIQKMAKVGVKNPLacetylation[2]
416KMAKVGVKNPLFLLDacetylation[2]
427FLLDEIDKMSSDMRGacetylation[2]
509AKRHLLPKQIERNALacetylation[1]
550GLEREISKLCRKAVKacetylation[2]
557KLCRKAVKQLLLDKSacetylation[2]
563VKQLLLDKSLKHIEIacetylation[2]
566LLLDKSLKHIEINGDacetylation[1, 2]
623ACVPGKGKLTYTGSLacetylation[1]
651VVRARAEKLGINPDFacetylation[1, 2]
661INPDFYEKRDIHVHVacetylation[2]
720VLPIGGLKEKLLAAHacetylation[2]
722PIGGLKEKLLAAHRGacetylation[2]
741VLIPFENKRDLEEIPacetylation[2]
761DLDIHPVKRIEEVLTacetylation[2]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, and UmuD. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system. 
Sequence Annotation
 DOMAIN 10 201 Lon.
 NP_BIND 356 363 ATP (By similarity).
 ACT_SITE 679 679
 ACT_SITE 722 722  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleotide-binding; Protease; Reference proteome; Serine protease; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 784 AA 
Protein Sequence
MNPERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSIRCL EAAMDHDKKI MLVAQKEAST 60
DEPGVNDLFT VGTVASILQM LKLPDGTVKV LVEGLQRARI SALSDNGEHF SAKAEYLESP 120
TIDEREQEVL VRTAISQFEG YIKLNKKIPP EVLTSLNSID DPARLADTIA AHMPLKLADK 180
QSVLEMSDVN ERLEYLMAMM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE 240
LGEMDDAPDE NEALKRKIDA AKMPKEAKEK AEAELQKLKM MSPMSAEATV VRGYIDWMVQ 300
VPWNARSKVK KDLRQAQEIL DTDHYGLERV KDRILEYLAV QSRVNKIKGP ILCLVGPPGV 360
GKTSLGQSIA KATGRKYVRM ALGGVRDEAE IRGHRRTYIG SMPGKLIQKM AKVGVKNPLF 420
LLDEIDKMSS DMRGDPASAL LEVLDPEQNV AFSDHYLEVD YDLSDVMFVA TSNSMNIPAP 480
LLDRMEVIRL SGYTEDEKLN IAKRHLLPKQ IERNALKKGE LTVDDSAIIG IIRYYTREAG 540
VRGLEREISK LCRKAVKQLL LDKSLKHIEI NGDNLHDYLG VQRFDYGRAD NENRVGQVTG 600
LAWTEVGGDL LTIETACVPG KGKLTYTGSL GEVMQESIQA ALTVVRARAE KLGINPDFYE 660
KRDIHVHVPE GATPKDGPSA GIAMCTALVS CLTGNPVRAD VAMTGEITLR GQVLPIGGLK 720
EKLLAAHRGG IKTVLIPFEN KRDLEEIPDN VIADLDIHPV KRIEEVLTLA LQNEPSGMQV 780
VTAK 784 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0009295; C:nucleoid; IBA:RefGenome.
 GO:0005524; F:ATP binding; IBA:RefGenome.
 GO:0004176; F:ATP-dependent peptidase activity; IDA:EcoliWiki.
 GO:0043565; F:sequence-specific DNA binding; IBA:RefGenome.
 GO:0004252; F:serine-type endopeptidase activity; IMP:EcoliWiki.
 GO:0033554; P:cellular response to stress; IEA:HAMAP.
 GO:0006515; P:misfolded or incompletely synthesized protein catabolic process; IBA:RefGenome.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR027543; Lon_bac.
 IPR004815; Lon_bac/euk-typ.
 IPR027065; Lon_Prtase.
 IPR027417; P-loop_NTPase.
 IPR008269; Pept_S16_C.
 IPR003111; Pept_S16_N.
 IPR008268; Peptidase_S16_AS.
 IPR015947; PUA-like_domain.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF00004; AAA
 PF02190; LON
 PF05362; Lon_C 
SMART
 SM00382; AAA
 SM00464; LON 
PROSITE
 PS01046; LON_SER 
PRINTS