CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018026
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase DDX54 
Protein Synonyms/Alias
 ATP-dependent RNA helicase DP97; DEAD box RNA helicase 97 kDa; DEAD box protein 54 
Gene Name
 DDX54 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
184ELALQTLKFTKELGKubiquitination[1]
383ARKINLAKFTLGKCSubiquitination[2]
418INYSFPAKGKLFLHRubiquitination[1]
420YSFPAKGKLFLHRVGubiquitination[1]
468LTLARPLKEPSGVAGubiquitination[1]
535PESIKRAKEMDLVGLubiquitination[1]
567LRLVDSIKNYRSRATubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors. 
Sequence Annotation
 DOMAIN 127 299 Helicase ATP-binding.
 DOMAIN 326 473 Helicase C-terminal.
 NP_BIND 140 147 ATP (By similarity).
 REGION 605 647 Interaction with nuclear receptors.
 MOTIF 96 124 Q motif.
 MOTIF 247 250 DEAD box.
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 39 39 Phosphoserine.
 MOD_RES 41 41 Phosphoserine.
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 644 644 Phosphoserine.
 MOD_RES 696 696 Phosphoserine.
 MOD_RES 698 698 Phosphoserine.
 MOD_RES 782 782 Phosphoserine.
 MOD_RES 788 788 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 881 AA 
Protein Sequence
MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG 60
PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS MGLSYPVFKG IMKKGYKVPT 120
PIQRKTIPVI LDGKDVVAMA RTGSGKTACF LLPMFERLKT HSAQTGARAL ILSPTRELAL 180
QTLKFTKELG KFTGLKTALI LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS 240
VEYVVFDEAD RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR 300
LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH AEYLTELLTT 360
QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR GLDIPLLDNV INYSFPAKGK 420
LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL LDLHLFLGRS LTLARPLKEP SGVAGVDGML 480
GRVPQSVVDE EDSGLQSTLE ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV 540
GLGLHPLFSS RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA 600
IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS EVVGRKRQRS 660
GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE GGAFEQQAAG AVLDLMGDEA 720
QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK IKTESGRYIS SSYKRDLYQK WKQKQKIDDR 780
DSDEEGASDR RGPERRGGKR DRGQAGASRP HAPGTPAGRV RPELKTKQQI LKQRRRAQKL 840
HFLQRGGLKQ LSARNRRRVQ ELQQGAFGRG ARSKKGKMRK RM 882 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:UniProtKB.
 GO:0030331; F:estrogen receptor binding; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0030520; P:intracellular estrogen receptor signaling pathway; TAS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006396; P:RNA processing; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR012541; DBP10CT.
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF08147; DBP10CT
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS