UniProt Accession

 NPD_ECOLI; P75960 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC74204.2; BAA35940.1 

Protein Name

 NAD-dependent protein deacylase 

Protein Synonyms/Alias

 Regulatory protein SIR2 homolog 

Gene Name

 cobB 

Gene Synonyms/Alias

 ycfY; b1120; JW1106 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
78	AAHLALAKLQDALGD	acetylation	[1]
231	VVPEFVEKLLKGLKA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. May also have NAD-dependent lysine demalonylase and desuccinylase activity (By similarity). Activates the enzyme acetyl-CoA synthetase by deacetylating 'Lys-609' in the inactive, acetylated form of the enzyme. May also modulate the activity of other propionyl-adenosine monophosphate (AMP)-forming enzymes (Probable). 

Sequence Annotation

 DOMAIN 1 237 Deacetylase sirtuin-type.
 NP_BIND 11 30 NAD (By similarity).
 NP_BIND 92 95 NAD (By similarity).
 NP_BIND 177 179 NAD (By similarity).
 NP_BIND 203 205 NAD (By similarity).
 ACT_SITE 110 110 Proton acceptor (By similarity).
 METAL 118 118 Zinc.
 METAL 137 137 Zinc.
 BINDING 55 55 Substrate (By similarity).
 BINDING 58 58 Substrate (By similarity).
 BINDING 221 221 NAD; via amide nitrogen (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; NAD; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 242 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0070403; F:NAD+ binding; IEA:HAMAP.
 GO:0034979; F:NAD-dependent protein deacetylase activity; IEA:HAMAP.
 GO:0036054; F:protein-malonyllysine demalonylase activity; IEA:InterPro.
 GO:0036055; F:protein-succinyllysine desuccinylase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:0006935; P:chemotaxis; IMP:EcoCyc.
 GO:0036047; P:peptidyl-lysine demalonylation; IEA:GOC.
 GO:0036049; P:peptidyl-lysine desuccinylation; IEA:GOC.
 GO:0006476; P:protein deacetylation; IDA:EcoCyc. 

Interpro

 IPR003000; Sirtuin.
 IPR026591; Sirtuin_cat_small_dom.
 IPR027546; Sirtuin_ClassIII.
 IPR026590; Ssirtuin_cat_dom. 

Pfam

 PF02146; SIR2 

SMART

  

PROSITE

 PS50305; SIRTUIN 

PRINTS