CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031066
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 460 
Protein Synonyms/Alias
 cDNA FLJ56994, highly similar to Zinc finger protein 460 
Gene Name
 ZNF460 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
76GTDPQSEKLHGKMSLubiquitination[1]
153EQILPRVKPYDCPECubiquitination[1, 2]
162YDCPECGKAFGKSKHubiquitination[1, 2]
190YKCLECGKDFNRRSHubiquitination[1, 2]
209QRTHNGDKPFVCSECubiquitination[1, 2]
237QRVHSGEKPFVCNECubiquitination[1, 2]
246FVCNECGKAFTYRSNubiquitination[1, 2]
259SNFVLHNKSHNEKKPubiquitination[1, 2]
264HNKSHNEKKPFACSEubiquitination[1, 2]
265NKSHNEKKPFACSECubiquitination[1]
296HTGERPFKCLECGKAubiquitination[3]
302FKCLECGKAFNCRSHubiquitination[1, 2, 4]
321ERIHTGEKPFVCSQCubiquitination[1]
349ERAHTGEKPFECKECubiquitination[1, 2]
354GEKPFECKECGKAFSubiquitination[1, 2]
358FECKECGKAFSIRKDubiquitination[2]
377FNIHTGEKPYECLQCubiquitination[1, 2, 4]
386YECLQCGKAFTRMSGubiquitination[1, 2]
405QWIHTGEKPYVCIQCubiquitination[1]
433FSIHTGEKPYECVECubiquitination[1, 2, 4]
442YECVECGKAFNRRSPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 521 AA 
Protein Sequence
MLETCGLLVA LGDSTKPETV EPIPSHLALP EEVSLQEQLA QGVPRYSYLG QAMDQDGPSE 60
MQEYFLRPGT DPQSEKLHGK MSLEHEGLAT ADGICSMMIQ NQVSPEDALY GFDSYGPVTD 120
SLIHEGENSY KFEEMFNENC FLVQHEQILP RVKPYDCPEC GKAFGKSKHL LQHHIIHTGE 180
KPYKCLECGK DFNRRSHLTR HQRTHNGDKP FVCSECGRTF NRGSHLTRHQ RVHSGEKPFV 240
CNECGKAFTY RSNFVLHNKS HNEKKPFACS ECGKGFYEST ALIQHFIIHT GERPFKCLEC 300
GKAFNCRSHL KQHERIHTGE KPFVCSQCGK AFTHYSTYVL HERAHTGEKP FECKECGKAF 360
SIRKDLIRHF NIHTGEKPYE CLQCGKAFTR MSGLTRHQWI HTGEKPYVCI QCGKAFCRTT 420
NLIRHFSIHT GEKPYECVEC GKAFNRRSPL TRHQRIHTAE KSHEPIQSGN VSCESTDLIQ 480
HSIIHTESSP VSAVNMETPS IAAHSSSLDI NGFIVEETLP L 521 
Gene Ontology
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS