CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019491
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein broad-minded 
Protein Synonyms/Alias
  
Gene Name
 BROMI 
Gene Synonyms/Alias
 C6orf170; C6orf171 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
168SSLNQSYKFCQGKLQubiquitination[1]
300RLLNEYQKEAPSFWIubiquitination[1]
747AGGIALKKSGFINELubiquitination[1]
814RNQDLPNKTEYSLREubiquitination[1]
981LCLKISDKQTEWIENubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Required for high-level Shh responses in the developing neural tube. Together with CDK20, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to Shh signaling (By similarity). 
Sequence Annotation
 DOMAIN 1119 1242 Rab-GAP TBC.  
Keyword
 Alternative splicing; Cell projection; Cilium; Complete proteome; Cytoplasm; Developmental protein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1257 AA 
Protein Sequence
MAHFSSEDQA MLQAMLRRLF QSVKEKITGA PSLECAEEIL LHLEETDENF HNYEFVKYLR 60
QHIGNTLGSM IEEEMEKCTS DRNQGEECGY DTVVQQVTKR TQESKEYKEM MHYLKNIMIA 120
VVESMINKFE EDETRNQERQ KKIQKEKSHS YRTDNCSDSD SSLNQSYKFC QGKLQLILDQ 180
LDPGQPKEVR YEALQTLCSA PPSDVLNCEN WTTLCEKLTV SLSDPDPVFS DRILKFCAQT 240
FLLSPLHMTK EIYTSLAKYL ESYFLSRENH IPTLSAGVDI TNPNMTRLLK KVRLLNEYQK 300
EAPSFWIRHP EKYMEEIVES TLSLLTVKHN QSHVVSQKIL DPIYFFALVD TKAVWFKKWM 360
HAHYSRTTVL RLLETKYKSL VTTAIQQCVQ YFEMCKTRKA DETLGHSKHC RNKQKTFYYL 420
GQELQYIYFI HSLCLLGRLL IYKQGRKLFP IKLKNKKGLV SLIDLLVLFT QLIYYSPSCP 480
KMTSAAHSEN YSPASMVTEV LWILSDQKEC AVECLYNNIV IETLLQPIHN LMKGNEASPN 540
CSETALIHIA GILARIASVE EGLILLLYGA NMNSSEESPT GAHIIAQFSK KLLDEDISIF 600
SGSEMLPVVK GAFISVCRHI YSTCEGLQVL ITYNLHESIA KAWKKTSLLS ERIPTPVEGS 660
DSVSSVSQES QNIMAWEDNL LDDLLHFAAT PKGLLLLQRT GAINECVTFI FNRYAKKLQV 720
SRHKKFGYGV LVTRVASTAA GGIALKKSGF INELITELWS NLEYGRDDVR VTHPRTTPVD 780
PIDRSCQKSF LALVNLLSYP AIYELVRNQD LPNKTEYSLR EVPTCVIDII DRLIILNSEA 840
KIRSLFNYEQ SHIFGLRLLS VICCDLDTLL LLEAQYQVSE MLLNAQEENI LEISESHRDF 900
IIDGLSVERN HVLVRINLVG GPLERILPPR LLEKSDNPYP WPMFSSYPLP NCYLSDITRN 960
AGIKQDNDLD KLLLCLKISD KQTEWIENCQ RQFCKMMKAK PDIISGEALI ELLEKFVLHL 1020
TESPSECYFP SVEYTATDAN VKNESLSSVQ QLGIKMTVRY GKFLSLLKDG AENDLTWVLK 1080
HCERFLKQQQ TSIKSSLLCL QGNYAGHDWF VSSLFMIMLG DKEKTFQFLH QFSRLLTSAF 1140
LWLPRLHISS YLPNDTVESG IHPVYFCSTH YIEMLLKAEL PLVFSAFHMS GFAPSQICLQ 1200
WITQCFWNYL DWIEICHYIA TCVFLGPDYQ VYICIAVFKH LQQDILQHTQ TQDLQVFLKE 1260
EALHGFRVSD YFEYMEILEQ NYRTVLLRDM RNIRLQST 1298 
Gene Ontology
 GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom.
 IPR005373; UPF0183. 
Pfam
  
SMART
  
PROSITE
 PS50086; TBC_RABGAP 
PRINTS