CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000433
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 High mobility group protein B3 
Protein Synonyms/Alias
 High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4 
Gene Name
 HMGB3 
Gene Synonyms/Alias
 HMG2A; HMG4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59MSGKEKSKFDEMAKAacetylation[1]
112SEFRPKIKSTNPGISubiquitination[2, 3, 4]
126SIGDVAKKLGEMWNNubiquitination[3]
145EKQPYITKAAKLKEKacetylation[1]
145EKQPYITKAAKLKEKubiquitination[5]
165ADYKSKGKFDGAKGPacetylation[6]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330
Functional Description
 Binds preferentially single-stranded DNA and unwinds double stranded DNA (By similarity). 
Sequence Annotation
 DNA_BIND 9 79 HMG box 1.
 DNA_BIND 93 161 HMG box 2.  
Keyword
 3D-structure; Chromosome; Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 200 AA 
Protein Sequence
MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF 60
DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI 120
GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV 180
EEEDEEEEEE EEEEEEEEDE 200 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008301; F:DNA binding, bending; ISS:UniProtKB.
 GO:0003690; F:double-stranded DNA binding; ISS:UniProtKB.
 GO:0006310; P:DNA recombination; ISS:UniProtKB.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc. 
Interpro
 IPR009071; HMG_box_dom.
 IPR017967; HMG_boxA_CS. 
Pfam
 PF09011; DUF1898
 PF00505; HMG_box 
SMART
 SM00398; HMG 
PROSITE
 PS00353; HMG_BOX_1
 PS50118; HMG_BOX_2 
PRINTS